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Adipose tissue\u2010derived mesenchymal stem cells (AT\u2010MSC) have shown promise in animal models to treat LSC deficiency (LSCD), but there are not studies showing their efficacy when primed with different media before transplantation. We cultured AT\u2010MSC with standard medium and media used to culture LSC for clinical application. We demonstrated that different media changed the AT\u2010MSC paracrine secretion showing different paracrine effector functions in an in vivo model of chemical burn and in response to a novel in vitro model of corneal inflammation by alkali induction. Treatment of LSCD with AT\u2010MSC changed the angiogenic and inflammatory cytokine profile of mice corneas. AT\u2010MSC cultured with the medium that improved their cytokine secretion, enhanced the anti\u2010angiogenic and anti\u2010inflammatory profile of the treated corneas. Those corneas also presented better outcome in terms of corneal transparency, neovascularization and histologic reconstruction. Priming human AT\u2010MSC with LSC specific medium can potentiate their ability to improve corneal wound healing, decrease neovascularization and inflammation modulating paracrine effector functions in an in vivo optimized rat model of LSCD.<\/jats:p>","DOI":"10.1111\/jcmm.16501","type":"journal-article","created":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T06:04:35Z","timestamp":1620281075000},"page":"5124-5137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Priming human adipose\u2010derived mesenchymal stem cells for corneal surface regeneration"],"prefix":"10.1111","volume":"25","author":[{"given":"N\u00faria","family":"Nieto\u2010Nicolau","sequence":"first","affiliation":[{"name":"CellTec\u2010UB Department of Cell Biology University of Barcelona Barcelona Spain"},{"name":"Barcelona Tissue Bank (BTB) Banc de Sang I Teixits (BST) Barcelona Spain"},{"name":"Institute of Biomedical Research IIB\u2010Sant 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