{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T21:28:14Z","timestamp":1772314094425,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucocorticoid\u2010induced osteonecrosis of the femoral head (GIONFH) is a common orthopaedic disease. GIONFH primarily manifests clinically as hip pain in the early stages, followed by the collapse of the femoral head, narrowing of the hip joint space and damage to the acetabulum, resulting in severely impaired mobility. However, the pathogenesis of GIONFH is not clearly understood. Recently, biomechanical forces and non\u2010coding RNAs have been suggested to play important roles in the pathogenesis of GIONFH. This study aimed to evaluate the role of biomechanical forced and non\u2010coding RNAs in GIONFH. We utilized an in vivo, rat model of GIONFH and used MRI, \u03bcCT, GIONFH\u2010TST (tail suspension test), GIONFH\u2010treadmill, haematoxylin and eosin staining, qRT\u2010PCR and Western blot analysis to analyse the roles of biomechanical forces and non\u2010coding RNAs in GIONFH. We used RAW264.7 cells and MC3T3E1 cells to verify the role of MALAT1\/miR\u2010329\u20105p\/PRIP signalling using a dual luciferase reporter assay, qRT\u2010PCR and Western blot analysis. The results demonstrated that MALAT1 and PRIP were up\u2010regulated in the femoral head tissues of GIONFH rats, RAW264.7 cells, and MC3T3E1 cells exposed to dexamethasone (Dex). Knockdown of MALAT1 decreased PRIP expression in rats and cultured cells and rescued glucocorticoid\u2010induced osteonecrosis of femoral head in rats. The dual luciferase reporter gene assay revealed a targeting relationship for MALAT1\/miR\u2010329\u20105p and miR\u2010329\u20105p\/PRIP in MC3T3E1 and RAW264.7 cells. In conclusion, MALAT1 played a vital role in the pathogenesis of GIONFH by binding to (\u2018sponging\u2019) miR\u2010329\u20105p to up\u2010regulate PRIP. Also, biomechanical forces aggravated the pathogenesis of GIONFH through MALAT1\/miR\u2010329\u20105p\/PRIP signalling.<\/jats:p>","DOI":"10.1111\/jcmm.16510","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T16:02:08Z","timestamp":1620057728000},"page":"5164-5176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The role of biomechanical forces and MALAT1\/miR\u2010329\u20105p\/PRIP signalling on glucocorticoid\u2010induced osteonecrosis of the femoral head"],"prefix":"10.1111","volume":"25","author":[{"given":"Guomin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedics Guizhou Provincial People\u2019s Hospital  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Joint Tianjin Hospital  Tianjin 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