{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:47:57Z","timestamp":1790376477675,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T00:00:00Z","timestamp":1624320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01GM1518B"],"award-info":[{"award-number":["01GM1518B"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Under healthy conditions, foot processes of neighbouring podocytes are interdigitating and connected by an electron\u2010dense slit diaphragm. Besides slit diaphragm proteins, typical adherens junction proteins are also found to be expressed at this cell\u2010cell junction. It is therefore considered as a highly specialized type of adherens junction. During podocyte injury, podocyte foot processes lose their characteristic 3D structure and the filtration slits typical meandering structure gets linearized. It is still under debate how this change of structure leads to the phenomenon of proteinuria. Using super\u2010resolution 3D\u2010structured illumination microscopy, we observed a spatially restricted up\u2010regulation of the tight junction protein claudin\u20105 (CLDN5) in areas where podocyte processes of patients suffering from minimal change disease (MCD), focal and segmental glomerulosclerosis (FSGS) as well as in murine nephrotoxic serum (NTS) nephritis and uninephrectomy DOCA\u2010salt hypertension models, were locally injured. CLDN5\/nephrin ratios in human glomerulopathies and NTS\u2010treated mice were significantly higher compared to controls. In patients, the CLDN5\/nephrin ratio is significantly correlated with the filtration slit density as a foot process effacement marker, confirming a direct association of local CLDN5 up\u2010regulation in injured foot processes. Moreover, CLDN5 up\u2010regulation was observed in some areas of high filtration slit density, suggesting that CLND5 up\u2010regulation preceded the changes of foot processes. Therefore, CLDN5 could serve as a biomarker predicting early foot process effacement.<\/jats:p>","DOI":"10.1111\/jcmm.16519","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T05:35:02Z","timestamp":1624340102000},"page":"7631-7641","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Super\u2010resolved local recruitment of CLDN5 to filtration slits implicates a direct relationship with podocyte foot process effacement"],"prefix":"10.1111","volume":"25","author":[{"given":"Florian","family":"Tesch","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology University Medicine Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1629-4982","authenticated-orcid":false,"given":"Florian","family":"Siegerist","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology University Medicine Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Hay","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology University Medicine Greifswald Greifswald Germany"},{"name":"Department of Mental and Physical Health and Preventive Medicine Section of Human Anatomy University of Campania \u201cLuigi Vanvitelli\u201d Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Artelt","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology University Medicine Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Daniel","sequence":"additional","affiliation":[{"name":"Department of Nephropathology Institute of Pathology University of Erlangen\u2010Nuremberg Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Amann","sequence":"additional","affiliation":[{"name":"Department of Nephropathology Institute of Pathology University of Erlangen\u2010Nuremberg Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Zimmermann","sequence":"additional","affiliation":[{"name":"Department of Urology University Medicine Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Kavvadas","sequence":"additional","affiliation":[{"name":"UMRS 1155 INSERM Sorbonne Universit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Grisk","sequence":"additional","affiliation":[{"name":"Institute for Physiology Medizinische Hochschule Brandenburg Theodor Fontane Neuruppin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Chadjichristos","sequence":"additional","affiliation":[{"name":"UMRS 1155 INSERM Sorbonne Universit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karlhans","family":"Endlich","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology University Medicine Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Chatziantoniou","sequence":"additional","affiliation":[{"name":"UMRS 1155 INSERM Sorbonne Universit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Endlich","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology University Medicine Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80057-X"},{"key":"e_1_2_9_3_1","first-page":"90","article-title":"Differentiation of epithelial foot processes and filtration slits: sequential appearance of occluding junctions, epithelial polyanion, and slit membranes in developing glomeruli","volume":"39","author":"Reeves W","year":"1978","journal-title":"Lab Invest"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.14.4829-4836.2001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/74166"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5438.312"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.10.3575-3582.2003"},{"key":"e_1_2_9_8_1","first-page":"43","article-title":"Alterations of the glomerular epithelium in acute aminonucleoside nephrosis. 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