{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T02:29:03Z","timestamp":1780108143584,"version":"3.54.0"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,5,2]],"date-time":"2021-05-02T00:00:00Z","timestamp":1619913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG8H0571"],"award-info":[{"award-number":["CMRPG8H0571"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study tested the hypothesis that combined therapy with human umbilical cord\u2010derived mesenchymal stem cells (HUCDMSCs) and hyperbaric oxygen (HBO) was superior to either one on preserving neurological function and reducing brain haemorrhagic volume (BHV) in rat after acute intracerebral haemorrhage (ICH) induced by intracranial injection of collagenase. Adult male SD rats (n\u00a0=\u00a030) were equally divided into group 1 (sham\u2010operated control), group 2 (ICH), group 3 (ICH +HUCDMSCs\/1.2\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> cells\/intravenous injection at 3h and days 1 and 2 after ICH), group 4 (ICH +HBO\/at 3\u00a0hours and days 1 and 2 after ICH) and group 5 (ICH +HUCDMSCs\u2010HBO), and killed by day 28 after ICH. By day 1, the neurological function was significantly impaired in groups 2\u20105 than in group 1 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001), but it did not differ among groups 2 to 5. By days 7, 14 and 28, the integrity of neurological function was highest in group 1, lowest in group 2 and significantly progressively improved from groups 3 to 5 (all <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). By day 28, the BHV was lowest in group 1, highest in group 2 and significantly lower in group 5 than in groups 3\/4 (all <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001). The protein expressions of inflammation (HMGB1\/TLR\u20102\/TLR\u20104\/MyD88\/TRAF6\/p\u2010NF\u2010\u03baB\/IFN\u2010\u03b3\/IL\u20101\u00df\/TNF\u2010\u03b1), oxidative stress\/autophagy (NOX\u20101\/NOX\u20102\/oxidized protein\/ratio of LC3B\u2010II\/LC3B\u2010I) and apoptosis (cleaved\u2010capspase3\/PARP), and cellular expressions of inflammation (CD14+, F4\/80+) in brain tissues exhibited an identical pattern, whereas cellular levels of angiogenesis (CD31+\/vWF+\/small\u2010vessel number) and number of neurons (NeuN+) exhibited an opposite pattern of BHV among the groups (all <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001). These results indicate that combined HUCDMSC\u2010HBO therapy offered better outcomes after rat ICH.<\/jats:p>","DOI":"10.1111\/jcmm.16577","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T05:02:15Z","timestamp":1620018135000},"page":"5640-5654","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Umbilical cord\u2010derived MSC and hyperbaric oxygen therapy effectively protected the brain in rat after acute intracerebral haemorrhage"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6305-5717","authenticated-orcid":false,"given":"Hon\u2010Kan","family":"Yip","sequence":"first","affiliation":[{"name":"Division of Cardiology Department of Internal Medicine Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine Kaohsiung Taiwan"},{"name":"Institute for Translational Research in Biomedicine Kaohsiung Chang Gung Memorial 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