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Hence, this study aims to investigate the key genes and relevant biological signalling pathways involved in human oocyte ageing. We isolated mRNA for single\u2010cell RNA sequencing from MII human oocytes donated by patients undergoing intracytoplasmic sperm injection. Nine RNA\u2010seq datasets were analyzed, which included 6 older patients(average 42.67\u00b12.25 years) and 3 younger patients (average 25.67\u00b12.08 years). 481 differentially expressed genes (DEGs) were identified, including 322 upregulated genes enriched in transcription, ubiquitination, epigenetic regulation, and cellular processes, and 159 downregulated genes enriched in ubiquitination, cell cycle, signalling pathway, and DNA repair. The STRING database was used to analyse protein\u2010protein interactions, and the Cytoscape software was used to identify hub genes. From these DEGs, 17 hub genes were identified including 12 upregulated genes (UBE2C, UBC, CDC34, UBR1, KIF11, ASF1B, PRC1, ESPL1, GTSE1, EXO1, UBA1, KIF4A) and 5 downregulated genes (UBA52, UBE2V2, SKP1, CCNB1, MAD2L1). The significant key biological processes that are associated with these hub genes include ubiquitin\u2010mediated proteolysis, ubiquitination\u2010related pathways, oocyte meiosis, and cell cycle. Among these, UBE2C may play a crucial role in human oocyte ageing.<\/jats:p>","DOI":"10.1111\/jcmm.16594","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T10:46:27Z","timestamp":1622025987000},"page":"6289-6303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Single\u2010cell transcriptome analysis of human oocyte ageing"],"prefix":"10.1111","volume":"25","author":[{"given":"Lihua","family":"Yuan","sequence":"first","affiliation":[{"name":"Shuguang Clinical Medical College Shanghai University of Traditional Chinese Medicine Shanghai China"},{"name":"Center of Reproductive Medicine Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Yin","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine Shuguang Hospital Affiliated to 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