{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:11:04Z","timestamp":1788876664711,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2021,6,13]],"date-time":"2021-06-13T00:00:00Z","timestamp":1623542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,13]],"date-time":"2021-06-13T00:00:00Z","timestamp":1623542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Transfusion\u2010related acute lung injury (TRALI) is a clinical syndrome which is associated with the formation of neutrophil extracellular trap (NET). Recent studies have demonstrated the roles of microRNAs (miRNAs) in the pathophysiological process of TRALI. Here, the study focused on the role of miR\u2010144 and the molecular mechanisms in NET\u2010induced TRALI. Up\u2010regulated miR\u2010144 and under\u2010expressed KLF2 were determined in patients with TRALI. In the mouse model of a two\u2010event TRALI induced by intraperitoneal injections with lipopolysaccharide and anti\u2010H\u20102Kd mAb, we determined expression patterns of miR\u2010144, Kr\u00fcppel\u2010like factor 2 (KLF2), chemokine (C\u2010X\u2010C motif) receptor 1 (CXCR1) and nuclear factor kappa\u2010B (NF\u2010kappaB) p65. The results confirmed that miR\u2010144 was highly expressed, while KLF2 was poorly expressed in mice with TRALI. Dual\u2010luciferase reporter gene assay identified that miR\u2010144 could target KLF2. Using gain\u2010 and loss\u2010of\u2010function approaches, we analysed the effects of miR\u2010144 and its interaction with KLF2 on TRALI. Enforced expression of miR\u2010144 was found to aggravate NET\u2010induced TRALI by down\u2010regulating KLF2 and activating the NF\u2010kappaB\/CXCR1 signalling pathway in TRALI mice. Collectively, miR\u2010144\u2010targeted inhibition of KLF2 and activation of NF\u2010kappaB\/CXCR1 are possible mechanisms responsible for NET\u2010caused TRALI. These findings aid in the development of therapeutic modalities for the treatment of TRALI.<\/jats:p>","DOI":"10.1111\/jcmm.16650","type":"journal-article","created":{"date-parts":[[2021,6,13]],"date-time":"2021-06-13T06:44:10Z","timestamp":1623566650000},"page":"6511-6523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["MiR\u2010144\u2010induced KLF2 inhibition and NF\u2010kappaB\/CXCR1 activation promote neutrophil extracellular trap\u2013induced transfusion\u2010related acute lung injury"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7121-0652","authenticated-orcid":false,"given":"Aiping","family":"Le","sequence":"first","affiliation":[{"name":"Departments of Blood Transfusion The First Affiliated Hospital of Nanchang University Nanchang 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