{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:51:27Z","timestamp":1792673487848,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T00:00:00Z","timestamp":1624406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MicroRNAs (miRNAs) serve as gene silencers involved in essential cell functions. The role of miR\u2010206 and E74\u2010like factor 3 (Elf3) has been identified in osteoarthritis (OA), while the effect of exosomal miR\u2010206 from bone marrow mesenchymal stem cells (BMSCs) in OA remains largely unknown. Thus, we aim to explore the role of exosomal miR\u2010206 from BMSCs in OA with the involvement of Elf3. BMSCs and BMSC\u2010derived exosomes (BMSC\u2010exos) were obtained and identified. OA mouse models were constructed by anterior cruciate ligament transection and then treated with BMSC\u2010exos or BMSC\u2010exos containing miR\u2010206 mimic\/inhibitor. The expression of miR\u2010206, Elf3, inflammatory factors, osteocalcin (OCN) and bone morphogenetic protein 2 (BMP2) in mouse femoral tissues was assessed. The pathological changes in mouse femur tissues were observed. The mouse osteoblasts were identified and treated with untransfected or transfected BMSC\u2010exos, and then, the expression of miR\u2010206, Elf3, OCN and BMP2 was determined. The alkaline phosphatase (ALP) activity, calcium deposition level, OCN secretion, proliferation, apoptosis and cell cycle arrest in osteoblasts were measured. MiR\u2010206 was down\u2010regulated while Elf3 was up\u2010regulated in OA animal and cellular models. Exosomal miR\u2010206 ameliorated inflammation and increased expression of OCN and BMP2 in mouse femoral tissues. Moreover, exosomal miR\u2010206 promoted ALP activity, calcium deposition level, OCN secretion and proliferation and inhibited apoptosis in OA osteoblasts. Overexpressed Elf3 reversed miR\u2010206 up\u2010regulation\u2010induced effects on OA osteoblasts. BMSC\u2010derived exosomal miR\u2010206 promotes proliferation and differentiation of osteoblasts in OA by reducing Elf3. Our research may provide novel targets for OA treatment.<\/jats:p>","DOI":"10.1111\/jcmm.16654","type":"journal-article","created":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T15:14:48Z","timestamp":1624461288000},"page":"7734-7745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Bone marrow mesenchymal stem cell\u2013derived exosomal miR\u2010206 promotes osteoblast proliferation and differentiation in osteoarthritis by reducing Elf3"],"prefix":"10.1111","volume":"25","author":[{"given":"Yijiang","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Orthopaedic The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province  Wenzhou 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