{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:41:57Z","timestamp":1791204117312,"version":"4.2.0"},"reference-count":45,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T00:00:00Z","timestamp":1623801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T00:00:00Z","timestamp":1623801600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81904234"],"award-info":[{"award-number":["81904234"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009601","name":"Beijing Municipal Administration of Hospitals","doi-asserted-by":"publisher","award":["DFL20151301"],"award-info":[{"award-number":["DFL20151301"]}],"id":[{"id":"10.13039\/501100009601","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The prethrombotic state (PTS) is a possible cause of recurrent spontaneous abortion (RSA). The aim of this study was to identify serum biomarkers for the detection of RSA with PTS (PSRSA). A Quantibody array 440 was used to screen novel serum\u2010based biomarkers for PSRSA\/NRSA (RSA without PTS). Proteins differentially expressed in PSRSA were analysed using bioinformatics methods and subjected to a customized array and enzyme\u2010linked immunosorbent assay (ELISA) validation. We used receiver operating characteristic to calculate diagnostic accuracy, and machine learning methods to establish a biomarker model for evaluation of the identified targets. 20 targets were selected for validation using a customized array, and seven targets via ELISA. The decision tree model showed that IL\u201024 was the first node and eotaxin\u20103 was the second node distinguishing the PSRSA and NRSA groups (an accuracy rate of 100% and an AUC of 1). Epidermal growth factor (EGF) as the node distinguished the PSRSA and NC groups (an accuracy rate of 100% and an AUC of 1). EGF as the node distinguished the NRSA and NC groups (an accuracy rate of 96.5% and an AUC of 0.998). Serum DNAM\u20101, BAFF, CNTF, LAG\u20103, IL\u201024, Eotaxin\u20103 and EGF represent a panel of promising diagnostic biomarkers to detect the PSRSA.<\/jats:p>","DOI":"10.1111\/jcmm.16671","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T06:34:13Z","timestamp":1623825253000},"page":"6679-6694","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Extensive serum biomarker analysis in the prethrombotic state of recurrent spontaneous abortion"],"prefix":"10.1111","volume":"25","author":[{"given":"Ying","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingwei","family":"Xin","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingshang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodan","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqin","family":"He","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2503-3285","authenticated-orcid":false,"given":"Chenghong","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2020.05.016"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075759"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/7495161","article-title":"The Efficacy of Traditional Chinese Medicine Shoutai Pill combined with Western Medicine in the first trimester of pregnancy in women with unexplained recurrent spontaneous abortion: a systematic review and meta\u2010analysis","volume":"2020","author":"Li HF","year":"2020","journal-title":"Biomed Res Int"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.908832"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000484297"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.12816\/0003333"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029616675967"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901073400102"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029609335518"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0044-100919"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6890"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.16766\/j.cnki.issn.1674\u20104152.2012.03.019"},{"key":"e_1_2_10_14_1","first-page":"137","volume-title":"Pre\u2010diffusive Intravascular Coagulation (pre\u2010DIC), the Diagnostic Reference Standards Developed by the Seventh National Conference on Thrombosis and Hemostasis. 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