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Puerarin (PUE) possesses excellent neuroprotective effects by suppressing the NF\u2010\u03baB pathway and activating the PI3K\/Akt signal, but its role and related mechanisms in ICH\u2010induced early brain injury (EBI) remain unclear. In this study, we intended to observe the effects of PUE and molecular mechanisms on ICH\u2010induced EBI. ICH was induced in rats by collagenase IV injection. PUE was intraperitoneally administrated alone or with simultaneously intracerebroventricular injection of LY294002 (a specific inhibitor of the PI3K\/Akt signal). Neurological deficiency, histological impairment, brain edema, hematoma volume, blood\u2013brain barrier destruction, and brain cell apoptosis were evaluated. Western blot, immunohistochemistry staining, reactive oxygen species (ROS) measurement, and enzyme\u2010linked immunosorbent assay were performed. PUE administration at 50\u00a0mg\/kg and 100\u00a0mg\/kg could significantly reduce ICH\u2010induced neurological deficits and EBI. Moreover, PUE could notably restrain ICH\u2010induced upregulation of the NF\u2010\u03baB pathway, pro\u2010inflammatory cytokines, ROS level, and apoptotic pathway and activate the PI3K\/Akt signal. However, LY294002 delivery could efficaciously weaken these neuroprotective effects of PUE. Overall, PUE could attenuate ICH\u2010induced behavioral defects and EBI possibly by PI3K\/Akt signal stimulation\u2010mediated inhibition of the NF\u2010\u03baB pathway, and this made PUE a potential candidate as a promising therapeutic option for ICH\u2010induced EBI.<\/jats:p>","DOI":"10.1111\/jcmm.16679","type":"journal-article","created":{"date-parts":[[2021,6,27]],"date-time":"2021-06-27T21:37:12Z","timestamp":1624829832000},"page":"7809-7824","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Puerarin attenuates intracerebral hemorrhage\u2010induced early brain injury possibly by PI3K\/Akt signal activation\u2010mediated suppression of NF\u2010\u03baB pathway"],"prefix":"10.1111","volume":"25","author":[{"given":"Jun","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Zhujiang Hospital The Engineering Technology Research Center of Education Ministry of China The National Key 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