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Unfortunately, effective preventive and treatment measures for inflammatory osteolysis are limited and usually confuse the orthopedist. Astragalus polysaccharide (APS), the main extractive of Astragali Radix, has been widely used for treating inflammatory diseases. In the current study, in vitro and in vivo experimental results demonstrated that APS notably inhibited osteoclast formation and differentiation dose\u2010dependently. Moreover, we found that APS down\u2010regulated RANKL\u2010related osteoclastogenesis and levels of osteoclast marker genes, such as\n                    <jats:italic>NFATC1<\/jats:italic>\n                    ,\n                    <jats:italic>TRAP<\/jats:italic>\n                    ,\n                    <jats:italic>c\u2010FOS<\/jats:italic>\n                    and cathepsin K. Further underlying mechanism investigation revealed that APS attenuated activity of MAPK signalling pathways (eg ERK, JNK and p38) and ROS production induced by RANKL. Additionally, APS was also found to suppress LPS\u2010related inflammatory osteolysis by decreasing inflammatory factors' production in vivo. Overall, our findings demonstrate that APS effectively down\u2010regulates inflammatory osteolysis due to osteoclast differentiation and has the potential to become an effective treatment of the disorders associated with osteoclast.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.16683","type":"journal-article","created":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T04:42:49Z","timestamp":1622781769000},"page":"6800-6814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Astragalus polysaccharide attenuates LPS\u2010related inflammatory osteolysis by suppressing osteoclastogenesis by reducing the MAPK signalling pathway"],"prefix":"10.1111","volume":"25","author":[{"given":"Jianye","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The First Affiliated Hospital of Chongqing Medical University Chongqing China"},{"name":"Department of Orthopaedics Fuling Central Hospital of 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