{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:11:04Z","timestamp":1788876664336,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T00:00:00Z","timestamp":1623024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T00:00:00Z","timestamp":1623024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fractalkine (CX3CL1, FKN), a CX3C gene sequence inflammatory chemokine, has been found to have pro\u2010inflammatory and pro\u2010adhesion effects. Macrophages are immune cells with a critical role in regulating the inflammatory response. The imbalance of M1\/M2 macrophage polarization can lead to aggravated inflammation. This study attempts to investigate the mechanisms through which FKN regulates macrophage activation and the acute kidney injury (AKI) involved in inflammatory response induced by lipopolysaccharide (LPS) by using FKN knockout (FKN\u2010KO) mice and cultured macrophages. It was found that FKN and Wnt\/\u03b2\u2010catenin signalling have a positive interaction in macrophages. FKN overexpression inhibited LPS\u2010induced macrophage apoptosis. However, it enhanced their cell viability and transformed them into the M2 type. The effects of FKN overexpression were accelerated by activation of Wnt\/\u03b2\u2010catenin signalling. In the in vivo experiments, FKN deficiency suppressed macrophage activation and reduced AKI induced by LPS. Inhibition of Wnt\/\u03b2\u2010catenin signalling and FKN deficiency further mitigated the pathologic process of AKI. In summary, we provide a novel mechanism underlying activation of macrophages in LPS\u2010induced AKI. Although LPS\u2010induced murine AKI was unable to completely recapitulate human AKI, the positive interactions between FKN and Wnt\/\u03b2\u2010catenin signalling pathway may be a therapeutic target in the treatment of kidney injury.<\/jats:p>","DOI":"10.1111\/jcmm.16707","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T03:02:22Z","timestamp":1623207742000},"page":"6963-6975","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Fractalkine aggravates LPS\u2010induced macrophage activation and acute kidney injury via Wnt\/\u03b2\u2010catenin signalling pathway"],"prefix":"10.1111","volume":"25","author":[{"given":"Qiming","family":"Gong","sequence":"first","affiliation":[{"name":"Department of Nephrology Affiliated Hospital of Youjiang Medical University for Nationalities Baise China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Science laboratory Youjiang Medical University for Nationalities Baise China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuhong","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Nephrology Affiliated Hospital of Youjiang Medical University for Nationalities Baise China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2612-5317","authenticated-orcid":false,"given":"Yanwu","family":"You","sequence":"additional","affiliation":[{"name":"Department of Nephrology Affiliated Hospital of Youjiang Medical University for Nationalities Baise China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3790"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162111"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4785"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102567.2011.03409.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005\u2010016\u20100395\u20109"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(00)80438\u20109"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2018.11.011"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075\u2010017\u20101487\u20106"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9972"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-017-0471-6"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010018\u20100227\u20100"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010pathol\u2010020117\u2010044010"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.254"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1681\/asn.2017040391"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084852"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201745517"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2016.21"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.12703\/P6\u201013"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2016.08.006"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2448"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.18\u20100105"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2018.01.027"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005\u2010014\u20100275\u20100"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3573"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI94330"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01813"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7902841"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.005457"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201008711\u20106"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.16707","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.16707","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.16707","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:51:29Z","timestamp":1788875489000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.16707"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,7]]},"references-count":29,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1111\/jcmm.16707"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.16707","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,7]]},"assertion":[{"value":"2021-03-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}