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It is vital to identify the novel prognostic biomarkers and therapeutic targets to assist with the subsequent diagnosis and treatment plan to mitigate the expansion of COVID\u201019. Since angiotensin\u2010converting enzyme 2 (ACE2)\u2010positive cells are hosts for COVID\u201019, we focussed on this cell type to explore the underlying mechanisms of COVID\u201019. In this study, we identified that ACE2\u2010positive cells from the bronchoalveolar lavage fluid (BALF) of patients with COVID\u201019 belong to bronchial epithelial cells. Comparing with patients of COVID\u201019 showing severe symptoms, the antigen processing and presentation pathway was increased and 12 typical genes, HLA\u2010DRB5, HLA\u2010DRB1, CD74, HLA\u2010DRA, HLA\u2010DPA1, HLA\u2010DQA1, HSP90AA1, HSP90AB1, HLA\u2010DPB1, HLA\u2010DQB1, HLA\u2010DQA2, and HLA\u2010DMA, particularly HLA\u2010DPB1, were obviously up\u2010regulated in ACE2\u2010positive bronchial epithelial cells of patients with mild disease. We further discovered SDCBP was positively correlated with above 12 genes particularly with HLA\u2010DPB1 in ACE2\u2010positive bronchial epithelial cells of COVID\u201019 patients. Moreover, SDCBP may increase the immune infiltration of B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils and dendritic cells in different lung carcinoma. Moreover, we found the expression of SDCBP was positively correlated with the expression of antigen processing and presentation genes in post\u2010mortem lung biopsies tissues, which is consistent with previous discoveries. These results suggest that SDCBP has good potential to be further developed as a novel diagnostic and therapeutic target in the treatment of COVID\u201019.<\/jats:p>","DOI":"10.1111\/jcmm.16714","type":"journal-article","created":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T02:47:16Z","timestamp":1623898036000},"page":"7001-7012","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Single\u2010cell RNA sequencing identify SDCBP in ACE2\u2010positive bronchial epithelial cells negatively correlates with COVID\u201019 severity"],"prefix":"10.1111","volume":"25","author":[{"given":"Ding","family":"Ma","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Molecular Medicine National Institute of Healthcare Data Science at Nanjing University Medical School of Nanjing University Nanjing China"},{"name":"Department of Hepatobiliary Surgery The Affiliated Drum Tower Hospital of Nanjing University Medical 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