{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:28:07Z","timestamp":1791329287077,"version":"4.3.1"},"reference-count":32,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2021,9,19]],"date-time":"2021-09-19T00:00:00Z","timestamp":1632009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Atherosclerosis (AS) is the main aetiology of coronary heart disease, cerebral infarction and peripheral vascular disease in humans. Long\u2010noncoding RNA (LincRNA)\u2010p21 has been reported to participate in the development of AS. Therefore, this study was designed to investigate the mechanism of LincRNA\u2010p21 on suppressing the development of AS. We fed ApoE<jats:sup>\u2212\/\u2212<\/jats:sup> mice with a high\u2010fat diet to induce an AS mouse model where the lesion area of AS and the extent of lipid deposition were measured. The binding of LincRNA\u2010p21 and miR\u2010221 or miR\u2010221 and SIRT1 was measured using a dual luciferase reporter gene assay and RIP. Following loss\u2010 and gain\u2010 function assays, CCK8, EdU, Transwell assay and scratch test were performed to determine the biological processes of human aortic endothelial cells (HAECs). miR\u2010221 was highly expressed while SIRT1 was poorly expressed in AS. LincRNA\u2010p21 acted as a sponge for miR\u2010221. miR\u2010221 targeted and negatively regulated the expression of SIRT1. LincRNA\u2010p21 promoted the deacetylation of Pcsk9 by SIRT1 by competitively binding to miR\u2010221, whereby promoting HAEC proliferation, migration and tube formation. In conclusion, LincRNA\u2010p21 acted as a molecular sponge for miR\u2010221 to promote deacetylation of the promoter region of Pcsk9 by SIRT1, therefore preventing the development of AS.<\/jats:p>","DOI":"10.1111\/jcmm.16771","type":"journal-article","created":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T03:26:44Z","timestamp":1632108404000},"page":"9141-9153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["LincRNA\u2010p21 alleviates atherosclerosis progression through regulating the miR\u2010221\/SIRT1\/Pcsk9 axis"],"prefix":"10.1111","volume":"25","author":[{"given":"Haojie","family":"Wang","sequence":"first","affiliation":[{"name":"Thoracic &amp; Cardiovascular Surgery Huaihe Hospital of Henan University Kaifeng China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"He","sequence":"additional","affiliation":[{"name":"Thoracic &amp; Cardiovascular Surgery Huaihe Hospital 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