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Recent studies have shown that hydrogen sulphide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) ameliorates cardiac functions in db\/db mice, but its regulation on the formation of LDs in cardiac tissues is unclear. Db\/db mice were injected with NaHS (40\u00a0\u03bcmol\u00b7kg\n                    <jats:sup>\u20101<\/jats:sup>\n                    ) for twelve weeks. H9c2 cells were treated with high glucose (40\u00a0mmol\/L), oleate (200\u00a0\u00b5mol\/L), palmitate (200\u00a0\u00b5mol\/L) and NaHS (100\u00a0\u00b5mol\/L) for 48\u00a0hours. Plasmids for the overexpression of wild\u2010type Hrd1 and Hrd1 mutated at Cys115 were constructed. The interaction between Hrd1 and DGAT1 and DGAT2, the ubiquitylation level of DGAT1 and 2, the S\u2010sulfhydration of Hrd1 were measured. Exogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S ameliorated the cardiac functions, decreased ER stress and reduced the number of LDs in db\/db mice. Exogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S could elevate the ubiquitination level of DGAT 1 and 2 and increased the expression of Hrd1 in cardiac tissues of db\/db mice. The S\u2010sulfhydration of Hrd1 by NaHS enhanced the interaction between Hrd1 and DGAT1 and 2 to inhibit the formation of LD. Our findings suggested that H\n                    <jats:sub>2<\/jats:sub>\n                    S modified Hrd1 S\u2010sulfhydration at Cys115 to reduce the accumulation of LDs in cardiac tissues of db\/db mice.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.16781","type":"journal-article","created":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T07:45:06Z","timestamp":1632555906000},"page":"9154-9167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Hydrogen sulphide reduced the accumulation of lipid droplets in cardiac tissues of db\/db mice via Hrd1 S\u2010sulfhydration"],"prefix":"10.1111","volume":"25","author":[{"given":"Yu","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linxue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of 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