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The molecular weight (MW) of SNP was determined to be 9223\u00a0Da by high\u2010performance gel permeation chromatography analyses, and the structure of the SNP repeat units was determined to be \u21923,4\u2010\u03b2\u2010D\u2010GlcpNAC (1\u2192 and \u21924) \u2010\u03b1\u2010D\u2010Glcp (1\u2192 in the ratio of 15:1; \u21922) \u2010\u03b1 \u2010D\u2010Galp \u2010 (1\u2192 as a side chain; and \u03b2\u2010D\u2010Galp\u2010(1\u2192 and \u03b1\u2010 D\u2010Glcp \u2010 (1\u2192 as end groups by GC\u2010MS analysis and NMR assays. The effect of SNP on hepatoma HepG2\u2010bearing mice was analysed to verify its potential in the clinical treatment of liver cancer. A total of 90\u00a0male athymic nu\/nu mice were divided into therapeutic and preventive groups and fed with different amounts of SNP. The antitumour effect of SNP on HepG2\u2010bearing mice and mechanism of such were studied by analysing the tumour size, spleen index, thymus index, immune factors in the blood, tumour apoptosis factors, etc. The results suggest that SNP not only increased the index of immune organs in the body, but also enhanced the secretion of immune factors, including interleukin\u20102, interferon gamma and tumour necrosis factor\u2010alpha in the serum. SNP induced the apoptosis of tumour cells via the mitochondrial apoptosis pathway, which upregulated caspase\u20103, caspase\u20108, caspase\u20109 and BCL2\u2010associated X, but downregulated B\u2010cell lymphoma\u20102 and vascular endothelial growth factor protein expression. In conclusion, SNP inhibited tumour growth by enhancing immune function and inducing tumour cell apoptosis in HepG2\u2010bearing mice. Therefore, SNP may be further investigated as a promising candidate for future antitumour drugs.<\/jats:p>","DOI":"10.1111\/jcmm.16793","type":"journal-article","created":{"date-parts":[[2021,7,24]],"date-time":"2021-07-24T11:06:27Z","timestamp":1627124787000},"page":"8338-8351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Novel polysaccharide extracted from <i>Sipunculus nudus<\/i> inhibits HepG2 tumour growth in vivo by enhancing immune function and inducing tumour cell apoptosis"],"prefix":"10.1111","volume":"25","author":[{"given":"Jie","family":"Su","sequence":"first","affiliation":[{"name":"Key Laboratory of Cultivation and High\u2010value Utilization of Marine Organisms in Fujian Province Fisheries Research Institute of Fujian  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengyuan","family":"Liao","sequence":"additional","affiliation":[{"name":"Key Laboratory of 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