{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T12:56:28Z","timestamp":1780664188651,"version":"3.54.1"},"reference-count":32,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T00:00:00Z","timestamp":1626825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570365"],"award-info":[{"award-number":["81570365"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was aimed at investigating whether Elabela (ELA) gene therapy can promote angiogenesis in the treatment of myocardial infarction (MI). The fusion expression plasmid pAAV\u20103\u00a0\u00d7\u00a0Flag\/ELA\u201032 was successfully constructed using molecular cloning technique. The model of acute MI was established by ligating the left anterior descending coronary artery in mice. Adeno\u2010associated virus serotype 9 (AAV9) was injected into the surrounding myocardium and tail vein immediately after the model was established. AAV was injected again from the tail vein one week later. Compared with the MI+PBS (control) group, the serum N\u2010terminal pro\u2010brain natriuretic peptide (NT\u2010proBNP) concentration, and the values of left ventricular end\u2010diastolic diameter (LVDd) and left ventricular end\u2010systolic diameter (LVDs) of the MI+AAV\u2010ELA (gene therapy) group were significantly decreased, while the value of left ventricular ejection fraction was significantly increased at 2 and 4\u00a0weeks after operation. Compared with the control group, the expression of CD105 and vWF and the percentage of CD31\u2010 and Ki67\u2013co\u2010positive cells were significantly increased in the gene therapy group. Moreover, the expressions of apelin peptide jejunum (APJ) receptor, vascular endothelial growth factor (VEGF), VEGFR2, Jagged1 and Notch3 in the heart tissue around the infarction were up\u2010regulated in mice with gene therapy. The results suggest that ELA activates VEFG\/VEGFR2 and Jagged1\/Notch3 pathways through APJ to promote angiogenesis after myocardial infarction. ELA gene therapy may be used in the treatment of ischaemic cardiomyopathy in future.<\/jats:p>","DOI":"10.1111\/jcmm.16814","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T06:42:49Z","timestamp":1626936169000},"page":"8537-8545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Elabela gene therapy promotes angiogenesis after myocardial infarction"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2839-3331","authenticated-orcid":false,"given":"Liangli","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine Affiliated Nanjing Brain Hospital Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Affiliated Nanjing Brain Hospital Nanjing Medical 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