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The investigation of BAG1 isoforms expression and intracellular localization in B\u2010cell acute lymphoblastic leukaemia (B\u2010ALL) patient\u2010derived specimens revealed that BAG1 levels decrease during disease remission, compared to diagnosis, but drastically increase at relapse. In particular, at diagnosis both BAG1\u2010L and BAG1\u2010M isoforms are mainly nuclear, while during remission the localization pattern changes, having BAG1\u2010M almost exclusively in the cytosol indicating its potential cytoprotective role in B\u2010ALL. In addition, knockdown of BAG1\/BAG3 induces cell apoptosis and G1\u2010phase cell cycle arrest and, more intriguingly, shapes cell response to chemotherapy. BAG1\u2010depleted cells show an increased sensitivity to the common chemotherapeutic agents, dexamethasone or daunorubicin, and to the BCL2 inhibitor ABT\u2010737. Moreover, the BAG1 inhibitor Thio\u20102 induces a cytotoxic effect on RS4;11 cells both in vitro and in a zebrafish xenograft model and strongly synergizes with pan\u2010BCL inhibitors. Collectively, these data sustain BAG1 deregulation as a critical event in assuring survival advantage to B\u2010ALL cells.<\/jats:p>","DOI":"10.1111\/jcmm.16822","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T04:53:41Z","timestamp":1629176021000},"page":"9060-9065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["BAG1 down\u2010regulation increases chemo\u2010sensitivity of acute lymphoblastic leukaemia cells"],"prefix":"10.1111","volume":"25","author":[{"given":"Elena","family":"Mariotto","sequence":"first","affiliation":[{"name":"Department of Woman's and Child's Health, Haematology\u2010Oncology Clinic and Lab University of Padova Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Corallo","sequence":"additional","affiliation":[{"name":"Neuroblastoma Laboratory Fondazione Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza 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