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This research aimed to explore the hypothesis that GADD153\u00a0mediates apoptosis in keloid fibroblasts exposed to BTXA. BTXA significantly induced GADD153 protein and mRNA expression in keloid fibroblasts. Treatment with c\u2010Jun N\u2010terminal kinase (JNK) inhibitor SP600125, JNK small interfering RNA (siRNA) and tumour necrosis factor\u2010alpha (TNF\u2010\u03b1) antibodies reversed the BTXA\u2010induced GADD153 expression. BTXA enhanced the transcriptional activity of GADD153, whereas the GADD153\u00a0mutant plasmid, JNK siRNA and anti\u2010TNF\u2010\u03b1 antibody treatment abolished the BTXA\u2010induced transcriptional activity of GADD153. The addition of TNF\u2010\u03b1 to keloid fibroblasts markedly increased GADD153 protein expression. The addition of GADD153\u00a0siRNA, SP600125 and anti\u2010TNF\u2010\u03b1 antibodies reversed cell death and caspase 3 and 9 activity induced by BTXA.<\/jats:p>","DOI":"10.1111\/jcmm.16881","type":"journal-article","created":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T11:40:06Z","timestamp":1630582806000},"page":"9402-9410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The molecular mechanism of GADD153 in apoptosis of keloid fibroblasts exposed to botulinum toxin type A"],"prefix":"10.1111","volume":"25","author":[{"given":"Ming\u2010Shiuan","family":"Nien","sequence":"first","affiliation":[{"name":"Department of plastic surgery Peking University Shenzhen Hospital Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Pin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Translational Medicine Center Shin Kong Wu Ho Su Memorial Hospital Taipei 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