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Myeloid differentiation primary response protein 88 (MyD88) is a central adaptor protein for innate immune system Toll\u2010like receptors (TLR) and induces cytokine secretion during retinal disease. We recently demonstrated that inhibiting MyD88 in mouse models of retinal degeneration led to increased photoreceptor survival, which was associated with altered cytokines and increased neuroprotective microglia. However, the identity of additional molecular changes associated with MyD88 inhibitor\u2010induced neuroprotection is not known. In this study, we used isobaric tags for relative and absolute quantification (iTRAQ) labelling followed by LC\u2010MS\/MS for quantitative proteomic analysis on the <jats:italic>rd10<\/jats:italic>\u00a0mouse model of retinal degeneration to identify protein pathways changed by MyD88 inhibition. Quantitative proteomics using iTRAQ LC\u2010MS\/MS is a high\u2010throughput method ideal for providing insight into molecular pathways during disease and experimental treatments. Forty\u2010two proteins were differentially expressed in retinas from mice treated with MyD88 inhibitor compared with control. Notably, increased expression of multiple crystallins and chaperones that respond to cellular stress and have anti\u2010apoptotic properties was identified in the MyD88\u2010inhibited mice. These data suggest that inhibiting MyD88 enhances chaperone\u2010mediated retinal protection pathways. Therefore, this study provides insight into molecular events contributing to photoreceptor protection from modulating inflammation.<\/jats:p>","DOI":"10.1111\/jcmm.16893","type":"journal-article","created":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T12:44:37Z","timestamp":1632573877000},"page":"9533-9542","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative proteomic analysis after neuroprotective MyD88 inhibition in the retinal degeneration 10 mouse"],"prefix":"10.1111","volume":"25","author":[{"given":"Tal","family":"Carmy\u2010Bennun","sequence":"first","affiliation":[{"name":"Bascom Palmer Eye Institute University of Miami Miller School of Medicine Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciara","family":"Myer","sequence":"additional","affiliation":[{"name":"Bascom Palmer Eye Institute University of Miami Miller School of Medicine Miami FL 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