{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:07:43Z","timestamp":1790348863398,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue engineering (TE) of long tracheal segments is conceptually appealing for patients with inoperable tracheal pathology. In tracheal TE, stem cells isolated from bone marrow or adipose tissue have been employed, but the ideal cell source has yet to be determined. When considering the origin of stem cells, cells isolated from a source embryonically related to the trachea may be more similar. In this study, we investigated the feasibility of isolating progenitor cells from pleura and pericard as an alternative cells source for tracheal tissue engineering. Porcine progenitor cells were isolated from pleura, pericard, trachea and adipose tissue and expanded in culture. Isolated cells were characterized by PCR, RNA sequencing, differentiation assays and cell survival assays and were compared to trachea and adipose\u2010derived progenitor cells. Progenitor\u2010like cells were successfully isolated and expanded from pericard and pleura as indicated by gene expression and functional analyses. Gene expression analysis and RNA sequencing showed a stem cell signature indicating multipotency, albeit that subtle differences between different cell sources were visible. Functional analysis revealed that these cells were able to differentiate towards chondrogenic, osteogenic and adipogenic lineages. Isolation of progenitor cells from pericard and pleura with stem cell features is feasible. Although functional differences with adipose\u2010derived stem cells were limited, based on their gene expression, pericard\u2010 and pleura\u2010derived stem cells may represent a superior autologous cell source for cell seeding in tracheal tissue engineering.<\/jats:p>","DOI":"10.1111\/jcmm.16916","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T06:50:08Z","timestamp":1635835808000},"page":"10869-10878","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Isolation of multipotent progenitor cells from pleura and pericardium for tracheal tissue engineering purposes"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9342-1493","authenticated-orcid":false,"given":"Rayna","family":"de Wit","sequence":"first","affiliation":[{"name":"Department of Cardio\u2010thoracic surgery Radboud University Medical Center Nijmegen the 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