{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:25:57Z","timestamp":1790832357769,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T00:00:00Z","timestamp":1633824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010023","name":"Natural Science Research of Jiangsu Higher Education Institutions of China","doi-asserted-by":"publisher","award":["BE2019676"],"award-info":[{"award-number":["BE2019676"]}],"id":[{"id":"10.13039\/501100010023","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010023","name":"Natural Science Research of Jiangsu Higher Education Institutions of China","doi-asserted-by":"publisher","award":["BK20171347"],"award-info":[{"award-number":["BK20171347"]}],"id":[{"id":"10.13039\/501100010023","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberculosis (TB) remains a worldwide healthcare concern, and the exploration of the host\u2010pathogen interaction is essential to develop therapeutic modalities and strategies to control <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (M.tb). In this study, RNA sequencing (transcriptome sequencing) was employed to investigate the global transcriptome changes in the macrophages during the different strains of M.tb infection. THP\u20101 cells derived from macrophages were exposed to the virulent M.tb strain H37Rv (Rv) or the avirulent M.tb strain H37Ra (Ra), and the M.tb BCG vaccine strain was used as a control. The cDNA libraries were prepared from M.tb\u2010infected macrophages and then sequenced. To assess the transcriptional differences between the expressed genes, the bioinformatics analysis was performed using a standard pipeline of quality control, reference mapping, differential expression analysis, protein\u2010protein interaction (PPI) networks, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Q\u2010PCR and Western blot assays were also performed to validate the data. Our findings indicated that, when compared to BCG or M.tb H37Ra infection, the transcriptome analysis identified 66 differentially expressed genes in the M.tb H37Rv\u2010infected macrophages, out of which 36\u00a0genes were up\u2010regulated, and 30\u00a0genes were down\u2010regulated. The up\u2010regulated genes were associated with immune response regulation, chemokine secretion, and leucocyte chemotaxis. In contrast, the down\u2010regulated genes were associated with amino acid biosynthetic and energy metabolism, connective tissue development and extracellular matrix organization. The Q\u2010PCR and Western blot assays confirmed increased expression of pro\u2010inflammatory factors, altered energy metabolic processes, enhanced activation of pro\u2010inflammatory signalling pathways and increased pyroptosis in H37Rv\u2010infected macrophage. Overall, our RNA sequencing\u2010based transcriptome study successfully identified a comprehensive, in\u2010depth gene expression\/regulation profile in M.tb\u2010infected macrophages. The results demonstrated that virulent M.tb strain H37Rv infection triggers a more severe inflammatory immune response associated with increased tissue damage, which helps in understanding the host\u2010pathogen interaction dynamics and pathogenesis features in different strains of M.tb infection.<\/jats:p>","DOI":"10.1111\/jcmm.16980","type":"journal-article","created":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T04:04:36Z","timestamp":1633925076000},"page":"10504-10520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Comparative transcriptomic analysis of THP\u20101\u2010derived macrophages infected with <i>Mycobacterium tuberculosis<\/i> H37Rv, H37Ra and BCG"],"prefix":"10.1111","volume":"25","author":[{"given":"Wenyuan","family":"Pu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science Medical School of Nanjing University Nanjing 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