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Further analysis was conducted to discover novel lncRNAs associated with melanoma, and candidates were prioritized for their potential association with invasiveness or other metastasis\u2010related processes. In this sense, we found the intergenic lncRNA U73166 (ENSG00000230454) and decided to explore its effects in melanoma. For that, we silenced the lncRNA U73166 expression using shRNAs in a melanoma cell line. Next, we experimentally investigated its functions and found that migration and invasion had significantly decreased in knockdown cells, indicating an essential association of lncRNA U73166 for cancer processes. Additionally, using na\u00efve and vemurafenib\u2010resistant cell lines and data from a patient before and after resistance, we found that vemurafenib\u2010resistant samples had a higher expression of lncRNA U73166. Also, we retrieved data from the literature that indicates lncRNA U73166\u00a0may act as a mediator of RNA processing and cell invasion, probably inducing a more aggressive phenotype. Therefore, our results suggest a relevant role of lncRNA U73166 in metastasis development. We also pointed herein the lncRNA U73166 as a new possible biomarker or target to help overcome clinical vemurafenib resistance.<\/jats:p>","DOI":"10.1111\/jcmm.16987","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T09:41:13Z","timestamp":1642498873000},"page":"671-683","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Upregulation of the novel lncRNA U731166 is associated with migration, invasion and vemurafenib resistance in melanoma"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2462-7767","authenticated-orcid":false,"given":"\u00c1damo Davi Di\u00f3genes","family":"Siena","sequence":"first","affiliation":[{"name":"Department of Genetics Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo (USP) Ribeir\u00e3o Preto Brazil"},{"name":"Center for Cell Based Therapy Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo 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