{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:43:28Z","timestamp":1788997408393,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019534","name":"Science Fund for Distinguished Young Scholars of Guangxi Province","doi-asserted-by":"publisher","award":["2020GXNSFAA297003"],"award-info":[{"award-number":["2020GXNSFAA297003"]}],"id":[{"id":"10.13039\/501100019534","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060072"],"award-info":[{"award-number":["82060072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study aimed to explore the potential hub genes and pathways of ischaemic cardiomyopathy (ICM) and to investigate the possible associated mechanisms. Two microarray data sets (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE5406\">GSE5406<\/jats:ext-link> and <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE57338\">GSE57338<\/jats:ext-link>) were downloaded from the Gene Expression Omnibus (GEO) database. The limma package was used to analyse the differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, Disease Ontology (DO) and Gene Ontology (GO) annotation analyses were performed. A protein\u2010protein interaction (PPI) network was set up using Cytoscape software. Significant modules and hub genes were identified by the Molecular Complex Detection (MCODE) app. Then, further functional validation of hub genes in other microarrays and survival analysis were performed to judge the prognosis. A total of 1065\u00a0genes were matched, with an adjusted <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05, and 17 were upregulated and 25 were downregulated with|log<jats:sub>2<\/jats:sub> (fold change)|\u22651.2. After removing the lengthy entries, GO identified 12 items, and 8 pathways were enriched at adjusted <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05 (false discovery rate, FDR set at &lt;0.05). Three modules with a score &gt;8 after MCODE analysis and MYH6 were ultimately identified. When validated in <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE23561\">GSE23561<\/jats:ext-link>, <jats:italic>MYH6<\/jats:italic> expression was lower in patients with CAD than in healthy controls (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE60993\">GSE60993<\/jats:ext-link> data suggested that <jats:italic>MYH6<\/jats:italic> expression was also lower in AMI patients (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In the <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE59867\">GSE59867<\/jats:ext-link> data set, <jats:italic>MYH6<\/jats:italic> expression was lower in CAD patients than in AMI patients and lower in heart failure (HF) patients than in non\u2010HF patients. However, there was no difference at different periods within half a year, and HF was increased when <jats:italic>MYH6<\/jats:italic> expression was low (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05\u20130.01). We performed an integrated analysis and validation and found that <jats:italic>MYH6<\/jats:italic> expression was closely related to ICM and HF. However, whether this marker can be used as a predictor in blood samples needs further experimental verification.<\/jats:p>","DOI":"10.1111\/jcmm.17015","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T07:47:03Z","timestamp":1635234423000},"page":"10736-10746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Identification of <i>MYH6<\/i> as the potential gene for human ischaemic cardiomyopathy"],"prefix":"10.1111","volume":"25","author":[{"given":"Jian\u2010Hong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cardiology Liuzhou People\u2019s Hospital  Liuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei\u2010Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oncology Liuzhou People\u2019s Hospital  Liuzhou 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