{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:47:39Z","timestamp":1788281259786,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Intrauterine adhesions (IUAs) severely hamper women's reproductive functions. Human amniotic mesenchymal stromal cell (hAMSC) transplantation is effective in treating IUAs. Here, we examined the function of Notch signalling in IUA treatment with hAMSC transplantation. Forty\u2010five Sprague\u2010Dawley female rats were randomly divided into the sham operation, IUA, IUA + E2, IUA + hAMSCs and IUA + hAMSCs + E2\u00a0groups. After IUA induction in the rats, hAMSCs promoted endometrial regeneration and repair via differentiation into endometrial epithelial cells. In all groups, the expression of key proteins in Notch signalling was detected in the uterus by immunohistochemistry. The results indicated Notch signalling activation in the hAMSCs and hAMSCs + E2\u00a0groups. We could also induce hAMSC differentiation to generate endometrial epithelial cells in vitro. Furthermore, the inhibition of Notch signalling using the AdR\u2010dnNotch1 vector suppressed hAMSC differentiation (assessed by epithelial and mesenchymal marker levels), whereas its activation using the AdR\u2010Jagged1 vector increased differentiation. The above findings indicate Notch signalling mediates the differentiation of hAMSCs into endometrial epithelial cells, thus promoting endometrial regeneration and repair; Notch signalling could have an important function in IUA treatment.<\/jats:p>","DOI":"10.1111\/jcmm.17023","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T12:56:21Z","timestamp":1635771381000},"page":"11002-11015","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Management of intrauterine adhesions using human amniotic mesenchymal stromal cells to promote endometrial regeneration and repair through Notch signalling"],"prefix":"10.1111","volume":"25","author":[{"given":"Jie","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayue","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Gou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congcong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingfeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benyuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjiang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nizhou","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiren","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University\u2010Town Hospital of Chongqing Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1332-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14708"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.08.039"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2016.10.018"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2013.10.026"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2009.10.009"},{"key":"e_1_2_9_8_1","first-page":"881","article-title":"Expert consensus on the diagnosis and management of intrauterine adhesions in China","volume":"50","author":"Chinese Society of Obstetrics and Gyneocology; Chinese Medical Association","year":"2015","journal-title":"Zhonghua Fu Chan Ke Za Zhi"},{"key":"e_1_2_9_9_1","first-page":"4941","article-title":"Transplantation of menstrual blood\u2010derived mesenchymal stem cells (MbMSCs) promotes the regeneration of mechanical injured endometrium","volume":"12","author":"Chen L","year":"2020","journal-title":"Am J Transl Res"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.02.096"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2020.06.012"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201900411"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000505691"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2017.02.003"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2010.12.016"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2020.12.008"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.205260"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-015-0062-3"},{"key":"e_1_2_9_19_1","first-page":"44","article-title":"Role of NF\u2010\u03baB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells","volume":"586","author":"Gou YT","year":"2020","journal-title":"J Third Mil Med Univ"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.095885"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2008.00963.x"},{"key":"e_1_2_9_22_1","article-title":"Increased expression of Jagged1\/Notch1 in the endometrium from patients with intrauterine adhesions","author":"Chen XH","year":"2014","journal-title":"Chin J Biochem Mol Biol"},{"key":"e_1_2_9_23_1","first-page":"2770","article-title":"Differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in vitro","volume":"20","author":"Qu LH","year":"2016","journal-title":"Chin J Tissue Eng Res"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2016-1002"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22031325"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-018-0087-7"},{"key":"e_1_2_9_27_1","first-page":"1660","article-title":"[In vivo tracing of human amniotic mesenchymal stem cells labeled with PKH26 in rat intrauterine adhesions model]","volume":"34","author":"Yang Y","year":"2018","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0700-5"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/GCO.0000000000000378"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2019.03.003"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-020-04012-1"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17023","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.17023","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T10:50:33Z","timestamp":1723027833000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.17023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":30,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/jcmm.17023"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.17023","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"2021-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}