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Inhibition of host cell apoptosis is the key to maintaining <jats:italic>Chlamydia<\/jats:italic> survival in vivo, and long noncoding RNAs (lncRNAs) play important roles in its developmental cycle and pathogenesis. However, it is not clear how lncRNAs regulate persistent <jats:italic>Chlamydia<\/jats:italic> infection. Here, using a microarray method, we identified 1718\u00a0lncRNAs and 1741\u00a0mRNAs differentially expressed in IFN\u2010\u03b3\u2010induced persistent <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> infection. Subsequently, 10 upregulated and 5 downregulated differentially expressed lncRNAs were verified by qRT\u2013PCR to confirm the reliability of the chip data. The GO and KEGG analyses revealed that differentially regulated transcripts were predominantly involved in various signalling pathways related to host immunity and apoptosis response. Targeted silencing of three lncRNAs (MIAT, ZEB1\u2010AS1 and IRF1) resulted in increased apoptosis rates. Furthermore, interference with lncRNA MIAT caused not only an obvious downregulation of the Bcl\u20102\/Bax ratio but also a marked release of cytochrome c, resulting in a significantly elevated level of caspase\u20103 activation. Meanwhile, MIAT was involved in the regulation of chlamydial development during the persistent infection. Collectively, these observations shed light on the enormous complex lncRNA regulatory networks involved in mitochondria\u2010mediated host cell apoptosis and the growth and development of <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jcmm.17069","type":"journal-article","created":{"date-parts":[[2021,12,3]],"date-time":"2021-12-03T08:53:20Z","timestamp":1638521600000},"page":"163-177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Chlamydia trachomatis<\/i> induces lncRNA MIAT upregulation to regulate mitochondria\u2010mediated host cell apoptosis and chlamydial development"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9452-0095","authenticated-orcid":false,"given":"Fangzhen","family":"Luo","sequence":"first","affiliation":[{"name":"Institute of Pathogenic Biology Hengyang Medical College Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control University of South China Hengyang China"},{"name":"Hunan Polytechnic of Environment and Biology Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yating","family":"Wen","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Hengyang Medical College Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control University of South China Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanhua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Hengyang Medical College Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control University of South China Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengmei","family":"Su","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Hengyang Medical College Hunan 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