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Our research investigated the role and mechanism of the m6A modification of lncRNA KCNQ1 overlapping transcript 1 (KCNQ1OT1) in Laryngeal squamous cell carcinoma (LSCC) progression. Microarray analysis was used to quantitatively detect the m6A apparent transcriptional modification level of lncRNA in LSCC tissue. Methylated RNA immunoprecipitation\u2010qPCR (MeRIP\u2010qPCR), in situ hybridization (ISH) and quantitative real\u2010time PCR (qRT\u2010PCR) were used to examine the m6A modification and expression of KCNQ1OT1. In addition, in vivo and in vitro experiments have tested the effects of KCNQ1OT1\u00a0knockdown on the proliferation, invasion and metastasis of LSCC. Mechanically, we found the N6\u2010methyladenosine (m6A) demethylase ALKBH5\u00a0mediates KCNQ1OT1 expression via an m6A\u2010YTHDF2\u2010dependent manner and KCNQ1OT1 could directly bind to HOXA9 to further regulate the proliferation, invasion and metastasis of LSCC cells. In general, our research indicates that ALKBH5\u2010mediated m6A modification of KCNQ1OT1 triggers the development of LSCC via upregulation of HOXA9.<\/jats:p>","DOI":"10.1111\/jcmm.17091","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T04:45:43Z","timestamp":1638333943000},"page":"385-398","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["ALKBH5\u2010mediated m6A modification of lncRNA KCNQ1OT1 triggers the development of LSCC via upregulation of HOXA9"],"prefix":"10.1111","volume":"26","author":[{"given":"Yushan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingrui","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Kan","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4587-3297","authenticated-orcid":false,"given":"Yanan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linli","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery The Second Affiliated Hospital Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2004.10.031"},{"issue":"4","key":"e_1_2_9_3_1","first-page":"313","article-title":"Laryngeal cancer: epidemiological data from Northern Greece and review of the literature","volume":"17","author":"Markou K","year":"2013","journal-title":"Hippokratia"},{"key":"e_1_2_9_4_1","unstructured":"GLOBOCAN 2018: Estimated Cancer Incidence Mortality and Prevalence Worldwide in 2018. 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