{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T03:42:23Z","timestamp":1775187743083,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T00:00:00Z","timestamp":1642982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The inhibitor of growth family member 4 (<jats:italic>ING4<\/jats:italic>) is one of the ING family genes, serves as a repressor of angiogenesis or tumour growth and suppresses loss of contact inhibition. Oncostatin M (OSM) is a multifunctional cytokine that belongs to the interleukin (IL)\u20106\u00a0subfamily with several biological activities. However, the role of recombinant adenoviruses co\u2010expressing <jats:italic>ING4<\/jats:italic> and <jats:italic>OSM<\/jats:italic> (Ad\u2010ING4\u2010OSM) in anti\u2010tumour activity of laryngeal cancer has not yet been identified. Recombinant Ad\u2010ING4\u2010OSM was used to evaluate their combined effect on enhanced anti\u2010tumour activity in Hep\u20102 cells of laryngeal cancer in vivo. Moreover, in vitro function assays of co\u2010expression of Ad\u2010ING4\u2010OSM were performed to explore impact of co\u2010expression of Ad\u2010ING4\u2010OSM on biological phenotype of laryngeal cancer cell line, that is Hep\u20102 cells. In vitro, Ad\u2010ING4\u2010OSM significantly inhibited the growth, enhanced apoptosis, altered cell cycle with G1 and G2\/M phase arrest, and upregulated the expression of P21, P27, P53 and downregulated survivin in laryngeal cancer Hep\u20102 cells. Furthermore, in vivo functional experiments of co\u2010expressing of Ad\u2010ING4\u2010OSM demonstrated that solid tumours in the nude mouse model were significantly suppressed, and the co\u2010expressing Ad\u2010ING4\u2010OSM showed a significant upregulation expression of P21, P53, Bax and Caspase\u20103 and a downregulation of Cox\u20102, Bcl\u20102 and CD34. This study for the first time demonstrated the clinical value and the role of co\u2010expressing Ad\u2010ING4\u2010OSM in biological function of laryngeal cancer. This work suggested that co\u2010expressing Ad\u2010ING4\u2010OSM might serve as a potential therapeutic target for laryngeal cancer patients.<\/jats:p>","DOI":"10.1111\/jcmm.17192","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T04:54:50Z","timestamp":1643086490000},"page":"1556-1566","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enhanced effect of recombinant adenoviruses co\u2010expression of <i>ING4<\/i> and <i>OSM<\/i> on anti\u2010tumour activity of laryngeal cancer"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1998-3348","authenticated-orcid":false,"given":"Fuwei","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiachen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyu","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiping","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jicheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Institute College of Medicine Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiyin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Bengbu Medical College  Bengbu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jisheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.6618"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407158101"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh256"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.28422"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clbc.2020.11.011"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3870"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.4626"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12984"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.24.9739"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.107158"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.7.2847"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"2977","DOI":"10.4049\/jimmunol.139.9.2977","article-title":"Purification and characterization of cytostatic lymphokines produced by activated human T lymphocytes. 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