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The aetiological mechanisms contributing to impaired AH outflow, however, are poorly understood. Here, we identified the secreted form of vasorin, a transmembrane glycoprotein, as a common constituent of human AH by mass spectrometry and immunoblotting analysis. ELISA assay revealed a significant but marginal decrease in vasorin levels in the AH of primary open\u2010angle glaucoma patients compared to non\u2010glaucoma cataract patients. Human trabecular meshwork (HTM) cells were confirmed to express vasorin, which has been shown to possess anti\u2010apoptotic and anti\u2010TGF\u2010\u03b2 activities. Treatment of HTM cells with vasorin induced actin stress fibres and focal adhesions and suppressed TGF\u2010\u03b22\u2010induced SMAD2\/3 activation in HTM cells. Additionally, cobalt chloride\u2010induced hypoxia stimulated a robust elevation in vasorin expression, and vasorin suppressed TNF\u2010\u03b1\u2010induced cell death in HTM cells. Taken together, these findings reveal the importance of vasorin in maintenance of cell survival, inhibition of TGF\u2010\u03b2 induced biological responses in TM cells, and the decreasing trend in vasorin levels in the AH of glaucoma patients suggests a plausible role for vasorin in the pathobiology of ocular hypertension and glaucoma.<\/jats:p>","DOI":"10.1111\/jcmm.17229","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T09:07:03Z","timestamp":1645002423000},"page":"2063-2075","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Role of vasorin, an anti\u2010apoptotic, anti\u2010TGF\u2010\u03b2 and hypoxia\u2010induced glycoprotein in the trabecular meshwork cells\u00a0and glaucoma"],"prefix":"10.1111","volume":"26","author":[{"given":"Jin A.","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Duke University School of Medicine  Durham North 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