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Dysregulated circRNAs in HCC were identified through bioinformatics analysis of Gene Expression Omnibus data sets. Quantitative real\u2010time PCR (qRT\u2010PCR), Sanger sequencing, RNase R digestion and actinomycin D treatment were conducted to confirm the characterization of circRNAs. CCK\u20108, wound\u2010healing and Transwell assays were performed to assess the functional roles of Hsa_circ_0003945 (Circ_0003945) in HCC cell lines. Subcellular fractionation and fluorescence in situ hybridization (FISH) were performed to locate Circ_0003945 in HCC cells. Dual\u2010luciferase reporter assay was executed to verify the binding of Circ_0003945 to microRNAs (miRNAs) or the miRNAs to their target genes. In this study, we found that Circ_0003945 was upregulated in HCC tissue, and higher Circ_0003945 expression was positively correlated with tumour size and tumour stage. Furthermore, high plasma levels of circulating Circ_0003945 were confirmed in HCC patients compared with those in non\u2010HCC groups. The functional experiments revealed that overexpression or knockdown of Circ_0003945 promoted or attenuated tumour growth and migration, respectively. Mechanistically, Circ_0003945 might exert as a miR\u201034c\u20105p sponge to upregulate the expression of leucine\u2010rich repeat\u2010containing G protein\u2010coupled receptor 4 (LGR4), activating the \u03b2\u2010catenin pathway, and finally facilitating HCC progression. Additionally, a \u03b2\u2010catenin activator could reverse the effect of Circ_0003945\u00a0knockdown. In conclusion, Circ_0003945 exerts a tumour\u2010promoting role in HCC cells by regulating the miR\u201034c\u20105p\/LGR4\/\u03b2\u2010catenin axis, which may be a potential target for HCC therapy.<\/jats:p>","DOI":"10.1111\/jcmm.17243","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T09:06:26Z","timestamp":1645002386000},"page":"2218-2229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Hsa_circ_0003945 promotes progression of hepatocellular carcinoma by mediating miR\u201034c\u20105p\/LGR4\/\u03b2\u2010catenin axis activity"],"prefix":"10.1111","volume":"26","author":[{"given":"Li\u2010Hua","family":"Lyu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Zhongshan Hospital Fudan University  Shanghai 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