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However, the possible impact of CatB on osteoclastogenesis elevated by non\u2010eukaryotic ceramides is largely unknown. It was reported that a novel class of phosphoglycerol dihydroceramide (PGDHC), produced by the key periodontal pathogen\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    upregulated RANKL\u2010mediated osteoclastogenesis in vitro and in vivo. Therefore, the aim of this study was to evaluate a crosstalk between host CatB and non\u2010eukaryotic PGDHC on the promotion of osteoclastogenesis. According to a pulldown assay, high affinity between PGDHC and CatB was observed in RANKL\u2010stimulated RAW264.7 cells in vitro. It was also demonstrated that PGDHC promotes enzymatic activity of recombinant CatB protein ex vivo and in RANKL\u2010stimulated osteoclast precursors in vitro. Furthermore, no or little effect of PGDHC on the RANKL\u2010primed osteoclastogenesis was observed in male and female CatB\u2010knock out mice compared with their wild type counterparts. Altogether, these findings demonstrate that bacterial dihydroceramides produced by\n                    <jats:italic>P<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>gingivalis<\/jats:italic>\n                    elevate RANKL\u2010primed osteoclastogenesis via direct activation of intracellular CatB in OCPs.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.17299","type":"journal-article","created":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T03:39:38Z","timestamp":1650080378000},"page":"2841-2851","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Crosstalk between dihydroceramides produced by\n                    <i>Porphyromonas gingivalis<\/i>\n                    and host lysosomal cathepsin B in the promotion of 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