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The post\u2010mortem diagnosis of heroin\u2010related death could be an issue and usually rely on a combination of investigations, including the autopsy, histological and toxicological analysis. We conducted the present study to evaluate the correlation between the heroin concentration in biological fluids (peripheral blood, bile and urine) and the post\u2010mortem anti\u20106\u2010MAM antibody expression in various tissues (brain, heart, lung, liver and kidney) using immunohistochemical staining. A quantitative analysis of the immunohistochemical reaction was carried out. 45 cases of heroin\u2010related death investigated at the Forensic Pathology Institutes of the University of Rome, Foggia and Pisa were included. The control group was composed of 15 cases of death due to other causes, without brain lesions and negative toxicological analysis for drugs. We found a positive immunohistochemical reaction in different organs and it was related to the timing of heroin metabolization. No reaction was found in the control group. Our findings show that immunohistochemistry can be a valuable tool for the post\u2010mortem diagnosis of acute heroin abuse. A better understanding of the timing of heroin's metabolism can be useful in the forensic field and for future therapeutic applications.<\/jats:p>","DOI":"10.1111\/jcmm.17351","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T16:52:46Z","timestamp":1659459166000},"page":"4666-4677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["6\u2010Monoacetylmorphine\u2010antibody distribution in tissues from heroin\u2010related death cases: An experimental study to investigate the distributive response"],"prefix":"10.1111","volume":"26","author":[{"given":"Aniello","family":"Maiese","sequence":"first","affiliation":[{"name":"Institute of Legal Medicine Department of Surgical Pathology Medical, Molecular and Critical Area University of Pisa Ospedale Santa Chiara  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaele","family":"La Russa","sequence":"additional","affiliation":[{"name":"Section of Legal Medicine Department of Clinical and Experimental Medicine University of Foggia Ospedale Colonnello D'Avanzo  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Chiara","family":"David","sequence":"additional","affiliation":[{"name":"Department of Public Security Health Central Directorate Research Center and Forensic Toxicology Laboratory Ministry of the Interior  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santina","family":"Cantatore","sequence":"additional","affiliation":[{"name":"Section of Legal Medicine Department of Clinical and Experimental Medicine University of Foggia Ospedale Colonnello D'Avanzo  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Chiara","family":"Manetti","sequence":"additional","affiliation":[{"name":"Institute of Legal Medicine Department of Surgical Pathology Medical, Molecular and Critical Area University of Pisa Ospedale Santa Chiara  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"De Matteis","sequence":"additional","affiliation":[{"name":"Department of Anatomical, Histological, Forensic and Orthopaedic Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Costantino","family":"Ciallella","sequence":"additional","affiliation":[{"name":"Department of Anatomical, Histological, Forensic and Orthopaedic Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Frati","sequence":"additional","affiliation":[{"name":"Department of Anatomical, Histological, Forensic and Orthopaedic Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1686-3236","authenticated-orcid":false,"given":"Vittorio","family":"Fineschi","sequence":"additional","affiliation":[{"name":"Department of Anatomical, Histological, Forensic and Orthopaedic Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2010.01.015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107517\u20102\u20102"},{"key":"e_1_2_9_4_1","unstructured":"WHO.Opioid overdose.https:\/\/www.who.int\/news\u2010room\/fact\u2010sheets\/detail\/opioid\u2010overdose. 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