{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:07:41Z","timestamp":1789474061003,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004448","name":"Aga Khan Foundation","doi-asserted-by":"publisher","award":["project n\u00ba330842553"],"award-info":[{"award-number":["project n\u00ba330842553"]}],"id":[{"id":"10.13039\/100004448","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,11]]},"abstract":"Abstract<\/jats:title>Sickle cell disease (SCD) is one of the most common genetic conditions worldwide. It can contribute up to 90% of under\u20105 mortality in sub\u2010Saharan Africa. Clinical manifestations are very heterogeneous, and the intestinal microbiome appears to be crucial in the modulation of inflammation, cell adhesion and induction of aged neutrophils, the main interveners of recurrent vaso\u2010occlusive crisis. Enterocyte injury, increased permeability, altered microbial composition and bacterial overgrowth have all been documented as microbial and pathophysiologic changes in the gut microbiome of SCD patients in recent studies. Our aim was to sequence the bacterial 16S rRNA gene in order to characterize the gut microbiome of Angolan children with SCA and healthy siblings as a control. A total of 72 stool samples were obtained from children between 3 and 14\u2009years old. Our data showed that the two groups exhibit some notable differences in microbiota relative abundance at different classification levels. Children with SCA have a higher number of the phylum Actinobacteria. As for the genus level, Clostridium<\/jats:italic> cluster XI bacteria was more prevalent in the SCA children, whereas the siblings had a higher abundance of Blautia, Aestuariispira, Campylobacter, Helicobacter, Polaribacter<\/jats:italic> and Anaerorhabdus<\/jats:italic>. In this study, we have presented the first microbiota analysis in an Angolan paediatric population with SCD and provided a detailed view of the microbial differences between patients and healthy controls. There is still much to learn before fully relying on the therapeutic approaches for gut modulation, which is why more research in this field is crucial to making this a reality.<\/jats:p>","DOI":"10.1111\/jcmm.17402","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T08:50:48Z","timestamp":1664355048000},"page":"5360-5368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Microbial gut evaluation in an angolan paediatric population with sickle cell disease"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0586-9154","authenticated-orcid":false,"given":"Mariana","family":"Delgadinho","sequence":"first","affiliation":[{"name":"H&TRC\u2010 Health & Technology Research Center, ESTeSL\u2010 Escola Superior de Tecnologia da Sa\u00fade Instituto Polit\u00e9cnico de Lisboa Lisbon 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