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However, the combined roles and molecular mechanism of IGF\u20101 and microRNAs (miRNAs) underlying PE remain unclear. Therefore, we first measured the expression of IGF\u20101, zinc finger E\u2010box binding homeobox 1 (ZEB1) and microRNA\u2010183 (miR\u2010183) expression in the placenta tissues of patients with PE by Western blot analysis and RT\u2010qPCR. Interactions among IGF\u20101, ZEB1 and miR\u2010183 were assessed by Western blot analysis, ChIP\u2010PCR and dual\u2010luciferase reporter gene assay. The effect of IGF\u20101 on the biological characteristics of trophoblast cells was investigated by CCK\u20108, colony formation assay and in vitro angiogenesis experiments after cells were transfected with si\u2010IGF\u20101. Finally, a mouse eclampsia model induced by knockdown of IGF\u20101 was established to confirm the in vitro effect of IGF\u20101 on PE. We found that IGF\u20101, ZEB1 and miR\u2010183 were highly expressed in the placental tissues of patients with PE. The knockdown of IGF\u20101 resulted in reduced proliferation and invasion of trophoblast cells and was accompanied by inhibited angiogenesis. ZEB1 was positively regulated by IGF\u20101 via ERK\/MAPK pathway, which in turn inhibited miR\u2010153 expression by binding to the miR\u2010183 promoter. The in vitro experiments further confirmed that IGF\u20101 knockdown could induce PE. To sum up, IGF\u20101 knockdown elevated expression of miR\u2010183 by downregulating ZEB1, thereby promoting deterioration of PE.<\/jats:p>","DOI":"10.1111\/jcmm.17403","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T12:34:16Z","timestamp":1680093256000},"page":"1179-1191","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Insulin\u2010like growth factor 1 ameliorates pre\u2010eclampsia by inhibiting zinc finger E\u2010box binding homeobox 1 by up\u2010regulation of <scp>microRNA<\/scp>\u2010183"],"prefix":"10.1111","volume":"27","author":[{"given":"Weisi","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Second Xiangya Hospital Central South University  Changsha 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