{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T19:01:27Z","timestamp":1780599687975,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:00:00Z","timestamp":1656374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472105"],"award-info":[{"award-number":["81472105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874008"],"award-info":[{"award-number":["81874008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mechanism underlying induction of periprosthetic osteolysis by wear particles remains unclear. In this study, cultured MLO\u2010Y4 osteocytic cells were exposed to different concentrations of titanium (Ti) particles. The results showed that Ti particles increased expression of the osteocytic marker SOST\/sclerostin in a dose\u2010dependent manner, accelerated apoptosis of MLO\u2010Y4 cells, increased the expression of IL\u20106, TNF\u2010\u03b1 and connexin 43. SOST silence alleviated the increase of MLO\u2010Y4 cells apoptosis, decreased the expression of IL\u20106, TNF\u2010\u03b1 and connexin 43 caused by Ti particles. The different co\u2010culture systems of MLO\u2010Y4 cells with MC3T3\u2010E1 osteoblastic cells were further used to observe the effects of osteocytic cells' changes induced by Ti particles on osteoblastic cells. MLO\u2010Y4 cells treated with Ti particles inhibited dramatically differentiation of MC3T3\u2010E1 cells mostly through direct cell\u2010to\u2010cell contact. SOST silence attenuated the inhibition effects of Ti\u2010induced MLO\u2010Y4 on MC3T3\u2010E1 osteoblastic differentiation, which ALP level and mineralization of MC3T3\u2010E1 cells increased and the expression of ALP, OCN and Runx2 increased compared to the Ti\u2010treated group. Taken together, Ti particles had negative effects on MLO\u2010Y4 cells and the impact of Ti particles on osteocytic cells was extensive, which may further inhibit osteoblastic differentiation mostly through intercellular contact directly. SOST\/sclerostin plays an important role in the process of mutual cell interaction. These findings may help to understand the effect of osteocytes in wear particle\u2010induced osteolysis.<\/jats:p>","DOI":"10.1111\/jcmm.17460","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T08:40:54Z","timestamp":1656405654000},"page":"4371-4385","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Osteocytic cells exposed to titanium particles increase sclerostin expression and inhibit osteoblastic cell differentiation mostly via direct cell\u2010to\u2010cell contact"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3511-9135","authenticated-orcid":false,"given":"Hao","family":"Chai","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Soochow University Suzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zai 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