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Intracavernosal pressure (ICP\/MAP) was measured in C57BL\/6 (WT) mice. Functional and immunoblotting assays were performed in corpora cavernosa (CC) strips from WT, NLRP3\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    and caspase\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice in the presence of ET\u20101 (100\u2009nM) and vehicle, MCC950, tiron, BAPTA AM, BQ123, or BQ788. ET\u20101 reduced the ICP\/MAP in WT mice, and MCC950 prevented the ET\u20101 effect. ET\u20101 decreased CC ACh\u2010, sodium nitroprusside (SNP)\u2010induced relaxation, and increased caspase\u20101 expression. BQ123 an ET\n                    <jats:sub>A<\/jats:sub>\n                    receptor antagonist reversed the effect. The ET\n                    <jats:sub>B<\/jats:sub>\n                    receptor antagonist BQ788 also reversed ET\u20101 inhibition of ACh and SNP relaxation. Additionally, tiron, BAPTA AM, and NLRP3 genetic deletion prevented the ET\u20101\u2010induced loss of ACh and SNP relaxation. Moreover, BQ123 diminished CC caspase\u20101 expression, while BQ788 increased caspase\u20101 and IL\u20101\u03b2 levels in a concentration\u2010dependent manner (100\u2009nM\u201310\u00a0\u03bcM). Furthermore, tiron and BAPTA AM prevented ET\u20101\u2010induced increase in caspase\u20101. In addition, BAPTA AM blocked ET\u20101\u2010induced ROS generation. In conclusion, ET\u20101\u2010induced erectile dysfunction depends on ET\n                    <jats:sub>A<\/jats:sub>\n                    \u2010 and ET\n                    <jats:sub>B<\/jats:sub>\n                    \u2010mediated activation of NLRP3 in mouse CC via Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010dependent ROS generation.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.17463","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T09:35:55Z","timestamp":1671010555000},"page":"1-14","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<scp>NLRP3<\/scp>\n                    activation contributes to endothelin\u20101\u2010induced erectile dysfunction"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4715-7318","authenticated-orcid":false,"given":"Rafael","family":"Sobrano Fais","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Ribeirao Preto Medical School University of Sao Paulo  Ribeirao Preto Brazil"},{"name":"Division of Pulmonary, Critical Care, and Sleep Medicine National Jewish Health  Denver Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5174-5710","authenticated-orcid":false,"given":"Rafael","family":"Menezes da Costa","sequence":"additional","affiliation":[{"name":"Special Academy Unit of Health Sciences Federal University of Goias  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Carvalho Mendes","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Ribeirao Preto Medical School University of Sao Paulo  Ribeirao Preto 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