{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:18:07Z","timestamp":1789474687804,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lung adenocarcinoma (LUAD) is the most challenging neoplasm to treat in clinical practice. Ankyrin repeat domain 49 protein (ANKRD49) is highly expressed in several carcinomas; however, its pattern of expression and role in LUAD are not known. Tissue microarrays, immunohistochemistry, <jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> test, Spearman correlation analysis, Kaplan\u2013Meier, log\u2010rank test, and Cox's proportional hazard model were used to analyse the clinical cases. The effect of ANKRD49 on the LUAD was investigated using CCK\u20108, clonal formation, would healing, transwell assays, and nude mice experiment. Expressions of ANKRD49 and its associated downstream protein molecules were verified by real\u2010time PCR, Western blot, immunohistochemistry, and\/or immunofluorescence analyses. ANKRD49 expression was highly elevated in LUAD. The survival rate and Cox's modelling analysis indicated that there may be an independent prognostic indicator for LUAD patients. We also found that ANKRD49 promoted the invasion and migration in both in <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> assays, through upregulating matrix metalloproteinase (MMP)\u20102 and MMP\u20109 activities via the P38\/ATF\u20102 signalling pathway Our findings suggest that ANKRD49 is a latent biomarker for evaluating LUAD prognosis and promotes the metastasis of A549 cells <jats:italic>via<\/jats:italic> upregulation of MMP\u20102 and MMP\u20109 in a P38\/ATF\u20102 pathway\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/jcmm.17464","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T06:39:48Z","timestamp":1656657588000},"page":"4401-4415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<scp>ANKRD49<\/scp> promotes the invasion and metastasis of lung adenocarcinoma via a <scp>P38<\/scp>\/<scp>ATF<\/scp>\u20102 signalling pathway"],"prefix":"10.1111","volume":"26","author":[{"given":"Yue\u2010hua","family":"Liu","sequence":"first","affiliation":[{"name":"School of Basic Medicine, Basic Medical Science Center Shanxi Medical University  Jinzhong China"},{"name":"Xi'an Jiaotong University\u2010Affiliated Honghui Hospital  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Basic Medical Science Center Shanxi Medical University  Jinzhong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai\u2010xue","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Basic Medical Science Center Shanxi Medical University  Jinzhong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The First Hospital Shanxi 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