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Our aim was to study the neutrophils profile on obesity and cardiovascular disease and its regulation by adipose tissue\u2010secretome and dapagliflozin. We have isolated neutrophils from patients undergoing open heart surgery (19 women and 51 men). Its migration activity was performed with culture\u2010transwell, transcriptional studies of proteolytic enzymes, adhesion molecules or receptors were analysed by real\u2010time PCR and proteomics (from 20 patients) analysis by TripleTOF mass spectrometer. Differentiated HL\u201060 (dHL\u201060) was used as a preclinical model on microfluidic for endothelial cells attaching assays and genes regulation with epicardial and subcutaneous fat secretomes from patients (3 women and 9 men) or dapagliflozin 1\u201310\u00a0\u03bcM treatments. The transcriptional and proteomics studies have determined higher levels of adhesion molecules in neutrophils from patients with obesity. The adhesion molecule CD11b levels were higher in those patients with the combined obesity and HFpEF factors (1.70\u2009\u00b1\u20090.06\u2009a.u. without obesity, 1.72\u2009\u00b1\u20090.04\u2009a.u. obesity or HFpEF without obesity and 1.79\u2009\u00b1\u20090.08\u2009a.u. obesity and HFpEF; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). While fat\u2010secretome induces its upregulation, dapagliflozin can modulated it. Because CD11b upregulation is associated with higher neutrophils migration and adhesion into endothelial cells, dapagliflozin might modulate this mechanism on patients with obesity and HFpEF.<\/jats:p>","DOI":"10.1111\/jcmm.17466","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T06:58:35Z","timestamp":1657609115000},"page":"4416-4427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Synergism between obesity and <scp>HFpEF<\/scp> on neutrophils phenotype and its regulation by adipose tissue\u2010molecules and <scp>SGLT2i<\/scp> dapagliflozin"],"prefix":"10.1111","volume":"26","author":[{"given":"Cristina","family":"Almengl\u00f3","sequence":"first","affiliation":[{"name":"Cardiology Group Health Research Institute  Santiago de Compostela 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