{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:44:24Z","timestamp":1790844264617,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["63611"],"award-info":[{"award-number":["63611"]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exosomes derived from solid tumour cells are involved in immune suppression, angiogenesis and metastasis; however, the role of leukaemia\u2010derived exosomes has less been investigated. Hence, changes in immune response\u2010related genes and human T cells apoptosis co\u2010incubated with exosomes isolated from patients' pre\u2010B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co\u2010incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT\u2010PCR. Apoptosis and caspase\u20103 and caspase\u20109 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs\u2010related cytokines, including TGF\u2010B and IL\u201010. The expression level of Th17\u2010related transcription factors (RoR\u03b3t) and interleukins (IL\u201017 and IL\u201023) decreased after this treatment. According to our findings, exosomes derived from ALL patients' sera carry immunosuppressive molecules, indicating the possible effect of exosomes as liquid biomarkers for cancer staging.<\/jats:p>","DOI":"10.1111\/jcmm.17482","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T09:34:52Z","timestamp":1657704892000},"page":"4566-4576","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Paediatric pre\u2010B acute lymphoblastic leukaemia\u2010derived exosomes regulate immune function in human T cells"],"prefix":"10.1111","volume":"26","author":[{"given":"Elham","family":"Gholipour","sequence":"first","affiliation":[{"name":"Student Research Committee Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houman","family":"Kahroba","sequence":"additional","affiliation":[{"name":"Departments of Toxicogenomics, GROW School for Oncology and Developmental Biology Maastricht University  Maastricht Netherlands"},{"name":"Center for Environmental Sciences Hasselt University  Hasselt Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasim","family":"Soltani","sequence":"additional","affiliation":[{"name":"Master of Science Neonatal Intensive Care Nursing, Faculity of Nursing and Midwifery Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Samadi","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Sarvarian","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajjad","family":"Vakili\u2010Samiani","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas Ali","family":"Hosein Pour Feizi","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3960-5712","authenticated-orcid":false,"given":"Mohammad\u00a0Sadegh","family":"Soltani\u2010Zangbar","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel","family":"Baghersalimi","sequence":"additional","affiliation":[{"name":"Pediatric Disease Research Center Guilan University of Medical Sciences  Rasht Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahram","family":"Darbandi","sequence":"additional","affiliation":[{"name":"Pediatric Disease Research Center Guilan University of Medical Sciences  Rasht Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliakbar","family":"Movassaghpour","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Talebi","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roza","family":"Motavalli","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Department of Molecular Medicine, Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3029-4172","authenticated-orcid":false,"given":"Amir","family":"Mehdizadeh","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolhassan","family":"Kazemi","sequence":"additional","affiliation":[{"name":"Medical Philosophy and History Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0099-6728","authenticated-orcid":false,"given":"Mehdi","family":"Yousefi","sequence":"additional","affiliation":[{"name":"Research Center for Integrative Medicine in Aging, Aging Research Institute Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,13]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"PuckettY ChanO.Cancer Acute Lymphocytic Leukemia (ALL).2017."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a034835"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijheh.2018.08.004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.614"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90867-7"},{"key":"e_1_2_9_7_1","volume-title":"Metabolic Polymorphisms and Susceptibility to Cancer, IARC Scienctific Publication No. 148","author":"Vineis P","year":"1999"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197804202981603"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","unstructured":"CristW CarrollA ShusterJ et al.Poor prognosis of children with Pre\u2010B acute lymphoblastic leukemia is associated with the t (1; 19)(q23; p13): a Pediatric Oncology Group Study;1990.","DOI":"10.1182\/blood.V76.1.117.117"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aat8549"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1115\/1.4035121"},{"issue":"6","key":"e_1_2_9_13_1","first-page":"5311","article-title":"Tumor progression mechanisms: insights from the central immune regulation of tissue homeostasis","volume":"17","author":"Lisovska N","year":"2019","journal-title":"Oncol Lett"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13040847"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118094"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2017.03.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2021.101446"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201211138"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2015.01.004"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20120265"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.1010.11.3"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2014.927432"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011469"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402237"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.07.005"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.271"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1684\/bdc.2010.1294"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10979-3"},{"issue":"8","key":"e_1_2_9_29_1","first-page":"e1261243","article-title":"Human tumor\u2010derived exosomes (TEX) regulate Treg functions via cell surface signaling rather than uptake mechanisms","volume":"6","author":"Muller L","year":"2017","journal-title":"Onco Targets Ther"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2014.15.6.2613"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3978"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1669"},{"issue":"8","key":"e_1_2_9_33_1","first-page":"1815","article-title":"Focus on exosomes: novel pathogenic components of leukemia","volume":"9","author":"Yang C","year":"2019","journal-title":"Am J Cancer Res"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.91"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.039743"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2020.11940"},{"key":"e_1_2_9_37_1","doi-asserted-by":"crossref","unstructured":"KahrobaH SamadiN MostafazadehM HejaziMS RezaM.Evaluating the presence of deregulated tumoral onco\u2010microRNAs in serum\u2010derived exosomes of gastric cancer patients as noninvasive diagnostic biomarkers;2021.","DOI":"10.34172\/bi.2021.22178"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-198-7_3"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20254"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-2819"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-12-4"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118871"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-2664"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.03.045"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17162"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-1489"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2022.01.012"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091463"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0251-y"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22105346"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v3.24641"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2118"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/416123"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-012-0628-4"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078091"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2007.03.006"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-3448"},{"issue":"1","key":"e_1_2_9_58_1","first-page":"47","article-title":"Antitumor activity and immune enhancement of murine interleukin\u201023 expressed in murine colon carcinoma cells","volume":"3","author":"Shan BE","year":"2006","journal-title":"Cell Mol Immunol"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1682"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0721-9"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2018.05.002"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-015-0181-x"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-019-0500-6"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6022"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900970"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3239"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2015.03.021"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17482","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.17482","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T13:17:05Z","timestamp":1692796625000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.17482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,13]]},"references-count":66,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1111\/jcmm.17482"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.17482","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,13]]},"assertion":[{"value":"2022-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}