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However, the detailed connection of this structure is troublesome and expensive to be identified. Low\u2010coverage whole genome sequencing (LCWGS) could not only detect the previously unknown chromosomal translocation, but also provide the breakpoint candidate small region (with an accuracy of \u00b1200 bases). Importantly, the sequencing cost of LCWGS is about US$300. Then, with the Sanger DNA sequencing, the precise breakpoint can be determined at a single base level. In our project, with LCWGS, BCR and ABL1 are successfully identified to be disrupted in three CML patients (at chr22:23,632,356 and chr9:133,590,450; chr22:23,633,748 and chr9:133,635,781; chr22: 23,631,831 and chr9:133,598,513, respectively). Due to the reconnection after chromosome breakage, classical fusion gene (BCR::ABL1) was found in bone marrow and peripheral blood. The precise breakpoints were helpful to investigate the pathogenic mechanism of CML and could better guide the classification of CML subtypes. This LCWGS method is universal and can be used to detect all diseases related to chromosome variation, such as solid tumours, liquid tumours and birth defects.<\/jats:p>","DOI":"10.1111\/jcmm.17500","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T05:25:20Z","timestamp":1659072320000},"page":"4721-4726","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Highly precise breakpoint detection of chromosome balanced translocation in chronic myelogenous leukaemia: Case series"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7478-3218","authenticated-orcid":false,"given":"Chuanchun","family":"Yang","sequence":"first","affiliation":[{"name":"Guangdong Medical University  Zhanjiang China"},{"name":"CheerLand Biological Technology Co., Ltd  Shenzhen 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