{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T21:15:30Z","timestamp":1768684530155,"version":"3.49.0"},"reference-count":11,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703412"],"award-info":[{"award-number":["81703412"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012243","name":"Foundation for Distinguished Young Talents in Higher Education of Guangdong","doi-asserted-by":"publisher","award":["2018KQNCX089"],"award-info":[{"award-number":["2018KQNCX089"]}],"id":[{"id":"10.13039\/501100012243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2020A1515111040"],"award-info":[{"award-number":["2020A1515111040"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conotoxins are promising neuropharmacological tools and drug candidates due to their high efficiency and specificity in targeting ion channels or neurotransmitter receptors. In this study, a novel O<jats:sub>2<\/jats:sub>\u2010superfamily conotoxin, Lt7b, was synthesized and its pharmacological functions were evaluated. Lt7b with three modified amino acids and three disulfide bonds was successfully synthesized. CD spectra showed that Lt7b had a typical \u03b1\u2010helix in the secondary structure. Patch clamp experiments on rat DRG neurons showed that Lt7b could significantly inhibit calcium currents with an IC<jats:sub>50<\/jats:sub> value of 856\u2009\u00b1\u200995\u2009nM. Meanwhile, 10\u00a0\u03bcM Lt7b could significantly increase the sodium currents by 77\u2009\u00b1\u20098%, but it had no obvious effects on the potassium currents in DRG neurons. In addition, patch clamp experiments on ion channel subtypes showed that 10\u00a0\u03bcM Lt7b could inhibit 7.0\u2009\u00b1\u20091.2%, 8.0\u2009\u00b1\u20091.5%, 4.6\u2009\u00b1\u20093.4%, and 9.5\u2009\u00b1\u20090.1% of the hCa<jats:sub>v<\/jats:sub>1.2, hCa<jats:sub>v<\/jats:sub>2.1, hCa<jats:sub>v<\/jats:sub>2.2, and hCa<jats:sub>v<\/jats:sub>3.2 currents, respectively, while it did not increase the rNa<jats:sub>v<\/jats:sub>1.7, rNa<jats:sub>v<\/jats:sub>1.8, hNa<jats:sub>v<\/jats:sub>1.5, hNa<jats:sub>v<\/jats:sub>1.7, and hNa<jats:sub>v<\/jats:sub>1.8 currents. Lt7b had no obvious toxicity to HaCaT and ND7\/23 cells up to 1\u2009mM and significantly increased the pain threshold at the testing time of 0.5\u20134\u00a0h in a dose\u2010dependent manner in the mouse hotplate assay. This novel conotoxin Lt7b may be a useful tool for ion channel studies and analgesic drug development.<\/jats:p>","DOI":"10.1111\/jcmm.17521","type":"journal-article","created":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T04:49:47Z","timestamp":1662094187000},"page":"5330-5334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Synthesis and evaluation of a novel analgesic conotoxin Lt7b that inhibits calcium currents and increases sodium currents"],"prefix":"10.1111","volume":"26","author":[{"given":"Yun","family":"Wu","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, The First Dongguan Affiliated Hospital Guangdong Medical University  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manyi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, NHC Key Laboratory of Nanobiological Technology Xiangya Hospital, Central South University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yubin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oncology, NHC Key Laboratory of Cancer Proteomics, State Local Joint Engineering Laboratory for Anticancer Drugs Xiangya Hospital, Central South University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of High Energy Physics Chinese Academy of Sciences  Shijingshan District China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0603-5223","authenticated-orcid":false,"given":"Maojun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oncology, NHC Key Laboratory of Cancer Proteomics, State Local Joint Engineering Laboratory for Anticancer Drugs Xiangya Hospital, Central South University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00207"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17060370"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr886"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22071057"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17050286"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2020.07.024"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins11020128"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7mb00511c"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.10.012"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2005.07.021"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/md15100313"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17521","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.17521","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17521","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T00:07:31Z","timestamp":1692662851000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.17521"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":11,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/jcmm.17521"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.17521","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9]]},"assertion":[{"value":"2021-08-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}