{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T03:33:15Z","timestamp":1769830395280,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T00:00:00Z","timestamp":1663891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we compared the inhibitory effects of recombinant oncolytic adenovirus (Ad\u2010apoptin\u2010hTERTp\u2010E1a, Ad\u2010VT) with that of doxorubicin (DOX), a first\u2010line chemotherapy drug, and tamoxifen (TAM), an endocrine therapy drug, on the proliferation of breast cancer cells. We found that Ad\u2010VT could effectively inhibit the proliferation of breast cancer cells (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01); the inhibition rate of Ad\u2010VT on normal mammary epithelial MCF\u201010A cells was less than 20%. DOX can effectively inhibit the proliferation of breast cancer cells and also has a strong inhibitory effect on MCF\u201010A cells (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01). TAM also has a strong inhibitory effect on breast cancer cells, among which the oestrogen\u2010dependent MCF\u20107 cell inhibition was stronger (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01), At higher concentrations, TAM also had a high rate of inhibition (&gt;70%) on the proliferation of MCF\u201010A cells. We also found that both recombinant adenovirus and both drugs could successfully induce tumour cell apoptosis. Further Western blot results showed that the recombinant adenovirus killed breast cancer cells through the endogenous apoptotic pathway. Analysis of the nude mouse subcutaneous breast cancer model showed that Ad\u2010VT significantly inhibited tumour growth (the luminescence rate of cancer cells was reduced by more than 90%) and improved the survival rate of tumour\u2010bearing mice (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01). Compared with DOX and TAM, Ad\u2010VT has a significant inhibitory effect on breast cancer cells, but almost no inhibitory effect on normal breast epithelial cells, and this inhibitory effect is mainly through the endogenous apoptotic pathway. These results indicate that Ad\u2010VT has significant potential as a drug for the treatment of breast cancer.<\/jats:p>","DOI":"10.1111\/jcmm.17549","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T08:45:53Z","timestamp":1663922753000},"page":"5222-5234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A comparative study of the ability of recombinant oncolytic adenovirus, doxorubicin and tamoxifen to inhibit the proliferation of breast cancer cells"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6727-3738","authenticated-orcid":false,"given":"Shanzhi","family":"Li","sequence":"first","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuoxin","family":"Li","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"},{"name":"Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun China"},{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6795-6088","authenticated-orcid":false,"given":"Yiquan","family":"Li","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jicheng","family":"Han","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Li","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningyi","family":"Jin","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"},{"name":"Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinbo","family":"Fang","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Li","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"},{"name":"Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangze","family":"Zhu","sequence":"additional","affiliation":[{"name":"Academiciann Workstation of Jilin Province Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S269880"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-020-06054-x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.suronc.2021.101531"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmn.2020.12.007"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD002748.pub4"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00524"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.9470"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2289\/fonc.2020.01026"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0963689719885078"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01797"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000004767"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2021.01.019"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics8040108"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.52028"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20204092"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abc6659"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12051139"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2019-000188"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines8120593"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2020.101006"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11809"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.111213"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23089"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9112438"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.46868"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines8120593"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17549","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.17549","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17549","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T00:06:32Z","timestamp":1692662792000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.17549"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,23]]},"references-count":27,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/jcmm.17549"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.17549","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,23]]},"assertion":[{"value":"2022-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}