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Fluorescence angiography was performed to examine the size of CNV lesions. Gene expression pattern of wound healing\u2010related components was examined. The effects of exogenous TNF\u03b1 on apoptosis of human retinal microvascular endothelial cells (HRMECs) and on the tube\u2010like structure of the cells were investigated in vitro. The results showed that Argon\u2010laser irradiation\u2010induced CNV was significantly larger in KO mice than WT mice on Day 21, but not at other timepoints. Lacking TNF\u03b1 increased neutrophil population in the lesion. The distribution of cleaved caspase3\u2010labelled apoptotic cells was more frequently observed in the laser\u2010irradiated tissue in a WT mouse as compared with a KO mouse. Exogenous TNF\u03b1 induced apoptosis of HRMECs and accelerated regression of tube\u2010like structure of HRMECs in cell culture. Taken together, TNF\u03b1 gene knockout delays the regression of laser\u2010induced CNV in mice. The mechanism underlying the phenotype might include the augmentation of neutrophil population in the treated tissue and attenuation of vascular endothelial cell apoptosis.<\/jats:p>","DOI":"10.1111\/jcmm.17562","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T05:22:21Z","timestamp":1663737741000},"page":"5315-5325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Delayed regression of laser\u2010induced choroidal neovascularization in <scp>TNF\u03b1<\/scp>\u2010null mice"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4192-6059","authenticated-orcid":false,"given":"Hiroki","family":"Iwanishi","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Wakayama Medical University  Wakayama 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