{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:10:28Z","timestamp":1787015428402,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>EN1 is well known as a transcription factor in other tumours, but its role in NPC is unclear. In this study, we first used bioinformatics to analyse GEO data to obtain the differentially expressed gene EN1, and subsequently verified that EN1 was highly expressed in nasopharyngeal carcinoma cells by tissue microarrays as well as cell lines. Further, we down\u2010regulated the expression of EN1 in cells for RNA sequencing. The analysis of sequencing results using KEGG and GO revealed significant changes in cell proliferation and cycle function after downregulation of EN1. Meanwhile, we found that cells underwent senescence after inhibition of EN1 under electron microscopy and the SA\u2010\u03b2\u2010gal assays. Based on the sequencing results, we verified that EN1 can promote the proliferation and cycle of NPC cells in cell function experiments and animal experiments. To investigate how EN1 affects cell senescence, we found that EN1 transcriptional regulation of COL22A1 regulated cell proliferation and cycle via CDK4\/6\u2010cyclin D1\u2010Rb signalling pathway by dual luciferase reporter, Immunoblotting and rescue experiment. Accordingly, we uncovered that EN1 could serve as a target for the regulation of senescence in NPC.<\/jats:p>","DOI":"10.1111\/jcmm.17575","type":"journal-article","created":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T04:17:05Z","timestamp":1664943425000},"page":"5473-5485","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Engrailed homeobox 1 transcriptional regulation of\n                    <scp>COL22A1<\/scp>\n                    inhibits nasopharyngeal carcinoma cell senescence through the\n                    <scp>G1<\/scp>\n                    \/S phase arrest"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4944-752X","authenticated-orcid":false,"given":"Mao\u2010Ling","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery The Second Affiliated Hospital of Chongqing Medical University  Chongqing 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