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Experimental evidences are in favour of an indirect pro\u2010haemostatic effect of desmopressin, but the exact mediator and its cellular origin are largely elusive. Here, we report partially hampered desmopressin response in a splenectomised severe haemophilia A\/Beta Thalassemia patient without any genetic variant relevant to his incomplete desmopressin response. To further investigate the role of the spleen in this phenomenon, the release of VWF from desmopressin\u2010treated human splenic endothelial cells was assessed in vitro. As a result, desmopressin induced the release of VWF from endothelial cells when the cells were co\u2010cultured with non\u2010classical (CD14<jats:sup>dim<\/jats:sup>\/CD16<jats:sup>++<\/jats:sup>), but not other subtypes of monocytes or PBMCs. This in vitro study which resembles close proximity of endothelial cells of sinusoids to monocyte reservoir reside in parenchyma of subcapsular red pulp of the spleen sheds a light upon the role of this highly vascularized VWF\u2010producing organ in driving indirect effect of desmopressin.<\/jats:p>","DOI":"10.1111\/jcmm.17606","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T14:57:01Z","timestamp":1670425021000},"page":"30-35","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pro\u2010haemostatic effect of <scp>DDAVP<\/scp> is partially derived through non\u2010classical (<scp>CD14<sup>dim<\/sup><\/scp>\/<scp>CD16<\/scp><sup>++<\/sup>) monocytes residing the spleen"],"prefix":"10.1111","volume":"27","author":[{"given":"Laleh","family":"Salarilak","sequence":"first","affiliation":[{"name":"Department of Genetics and Molecular Medicine, School of Medicine Zanjan University of Medical Sciences (ZUMS)  Zanjan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Pirdel","sequence":"additional","affiliation":[{"name":"Deputy of Research and Technology Ardabil University of Medical Sciences  Ardabil Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Dinmohammadi","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Medicine, School of Medicine Zanjan University of Medical Sciences (ZUMS)  Zanjan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Rokni\u2010Zadeh","sequence":"additional","affiliation":[{"name":"Zanjan Pharmaceutical Biotechnology Research Center Zanjan University of Medical Sciences (ZUMS)  Zanjan Iran"},{"name":"Department of Medical Biotechnology School of Medicine, Zanjan University of Medical Sciences (ZUMS)  Zanjan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renaud","family":"Lavend'homme","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehran","family":"Karimi","sequence":"additional","affiliation":[{"name":"Hematology Research Center Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Jacquemin","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5551-9377","authenticated-orcid":false,"given":"Tina","family":"Shahani","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Medicine, School of Medicine Zanjan University of Medical Sciences (ZUMS)  Zanjan Iran"},{"name":"Zanjan Pharmaceutical Biotechnology Research Center Zanjan University of Medical Sciences (ZUMS)  Zanjan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.7.2515"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-06-2072"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00604.2013"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110265"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041540307"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V60.6.1402.1402"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2006.03.020"},{"key":"e_1_2_9_9_1","first-page":"201","article-title":"Transplantation of spleen cells in patients with hemophilia A. 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