{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:45:55Z","timestamp":1762177555683,"version":"3.40.5"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T00:00:00Z","timestamp":1671494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971322"],"award-info":[{"award-number":["81971322"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Circular RNAs (circRNAs) are often found in eukaryocyte and have a role in the pathogenesis of a variety of human disorders. Our related research has shown the differential expression of circRNAs in periprosthetic osteolysis (PPOL). However, the involvement of circRNAs in the exact process is yet unknown. CircSLC8A1 expression was evaluated in clinical samples and human bone marrow mesenchymal stem cells (hBMSCs) in this investigation using quantitative real\u2010time PCR. In vitro and in vivo studies were conducted to explicate its functional role and pathway. We demonstrated CircSLC8A1 is involved in PPOL using gain\u2010 and loss\u2010of\u2010function methods. The association of CircSLC8A1 and miR\u2010144\u20103p, along with miR\u2010144\u20103p and RUNX1, was predicted using bioinformatics. RNA pull\u2010down and luciferase assays confirmed it. The impact of CircSLC8A1 in the PPOL\u2010mouse model was also investigated using adeno\u2010associated virus. CircSLC8A1 was found to be downregulated in PPOL patients' periprosthetic tissues. Overexpression of CircSLC8A1 promoted osteogenic differentiation (OD) and inhibited apoptosis of hBMSCs in vitro. The osteogenic markers of RUNX1, osteopontin (OPN) and osteocalcin (OCN) were significantly upregulated in hBMSCs after miR\u2010144\u20103p inhibitor was transferred. Mechanistic analysis demonstrated that CircSLC8A1 directly bound to miR\u2010144\u20103p and participated in PPOL through the miR\u2010144\u20103p\/RUNX1 pathway in hBMSCs. Micro\u2010CT and quantitative analysis showed that CircSLC8A1 markedly inhibited PPOL, and osteogenic markers (RUNX1, OPN and OCN) were significantly increased (P&lt;0.05) in the mice model. Our findings prove that Circ<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=6546\">SLC8A1<\/jats:ext-link> exerted a regulatory role in promoting osteogenic differentiation in hBMSCs, and CircSLC8A1\/miR\u2010144\u20103p\/RUNX1 pathway may provide a potential target for prevention of PPOL.<\/jats:p>","DOI":"10.1111\/jcmm.17633","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T07:58:01Z","timestamp":1671609481000},"page":"189-203","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Circular <scp>RNA CircSLC8A1<\/scp> contributes to osteogenic differentiation in <scp>hBMSCs<\/scp> via <scp>CircSLC8A1<\/scp>\/<scp>miR<\/scp>\u2010144\u20103p\/<scp>RUNX1<\/scp> in periprosthetic 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