{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:52:35Z","timestamp":1783605155860,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFC1703202"],"award-info":[{"award-number":["2017YFC1703202"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Streptococcus pneumoniae<\/jats:italic> (<jats:italic>S.\u00a0pneumoniae<\/jats:italic>) is a major causative agent of respiratory disease in patients and can cause respiratory distress and other symptoms in severe cases. Pneumolysin (PLY) is a pore\u2010forming toxin that induces host tissue injury and inflammatory responses. Sortase A (SrtA), a catalytic enzyme that anchors surface\u2010associated virulence factors, is critical for <jats:italic>S.\u00a0pneumoniae<\/jats:italic> virulence. Here, we found that the active ingredient of the Chinese herb <jats:italic>Scutellaria baicalensis<\/jats:italic>, wogonin, simultaneously inhibited the haemolytic activity of PLY and SrtA activity. Consequently, wogonin decreased PLY\u2010mediated cell damage and reduced SrtA\u2010mediated biofilm formation by <jats:italic>S.\u00a0pneumoniae<\/jats:italic>. Furthermore, our data indicated that wogonin did not affect PLY expression but directly altered its oligomerization, leading to reduced activity. Furthermore, the analysis of a mouse pneumonia model further revealed that wogonin reduced mortality in mice infected with <jats:italic>S.\u00a0pneumoniae<\/jats:italic> laboratory strain D39 and <jats:italic>S.\u00a0pneumoniae<\/jats:italic> clinical isolate E1, reduced the number of colony\u2010forming units in infected mice and decreased the W\/D ratio and levels of the inflammatory factors TNF\u2010\u03b1, IL\u20106 and IL\u20101\u03b2 in the lungs of infected mice. Thus, wogonin reduces <jats:italic>S.\u00a0pneumoniae<\/jats:italic> pathogenicity by inhibiting the dual targets PLY and SrtA, providing a treatment option for <jats:italic>S.\u00a0pneumoniae<\/jats:italic> infection<jats:italic>.<\/jats:italic><\/jats:p>","DOI":"10.1111\/jcmm.17684","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T06:19:20Z","timestamp":1675750760000},"page":"563-575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Wogonin attenuates the pathogenicity of <i>Streptococcus pneumoniae<\/i> by double\u2010target inhibition of Pneumolysin and Sortase A"],"prefix":"10.1111","volume":"27","author":[{"given":"Kuan","family":"Gu","sequence":"first","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhong","family":"Ding","sequence":"additional","affiliation":[{"name":"Affiliated Hospital to Changchun University of Chinese Medicine  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongtian","family":"Wang","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Sun","sequence":"additional","affiliation":[{"name":"Affiliated Hospital to Changchun University of Chinese Medicine  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhou","family":"Sun","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Yang","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese 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