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Preosteoblasts were differentiated actively to matured osteoblasts by intracellular PPM1A overexpression. However, it was unclear whether extracellular PPM1A contributes to the excessive bone\u2010forming activity in AS. Here, we confirmed that PPM1A and runt\u2010related transcription factor 2 (RUNX2) were increased in facet joints of AS. During osteoblasts differentiation, exogenous PPM1A treatment showed increased matrix mineralization in AS\u2010osteoprogenitor cells accompanied by induction of RUNX2 and factor forkhead box O1A (FOXO1A) protein expressions. Moreover, upon growth condition, exogenous PPM1A treatment showed an increase in RUNX2 and FOXO1A protein expression and a decrease in phosphorylation at ser256 of FOXO1A protein in AS\u2010osteoprogenitor cells, and positively regulated promoter activity of RUNX2 protein\u2010binding motif. Mechanically, exogenous PPM1A treatment induced the dephosphorylation of transcription factor FOXO1A protein and translocation of FOXO1A protein into the nucleus for RUNX2 upregulation. Taken together, our results suggest that high PPM1A concentration promotes matrix mineralization in AS via the FOXO1A\u2010RUNX2 pathway.<\/jats:p>","DOI":"10.1111\/jcmm.17685","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T09:10:46Z","timestamp":1675933846000},"page":"650-658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Extracellular <scp>PPM1A<\/scp> promotes mineralization of osteoblasts differentiation in ankylosing spondylitis via the <scp>FOXO1A\u2010RUNX2<\/scp> pathway"],"prefix":"10.1111","volume":"27","author":[{"given":"Subin","family":"Weon","sequence":"first","affiliation":[{"name":"Hanyang University Institute for Rheumatology Research (HYIRR)  Seoul Korea"},{"name":"Department of Translational Medicine, Graduate School of Biomedical Science and Engineering Hanyang University  Seoul 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