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Kaempferol is a flavonoid compound with broad anti\u2010inflammatory and antioxidant properties. The purpose of this study was to investigate the effect of kaempferol on melanogenesis in PIG1 normal human skin melanocytes and its response to oxidative stress. The effect of kaempferol on melanin synthesis in PIG1 normal human skin melanocytes was explored by measuring tyrosinase activity, melanin content, mRNA and protein expression of key enzymes and expression of related pathway proteins. The effects of kaempferol pretreatment on cell viability, apoptosis, ROS level and HO\u20101 protein level under H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> stimulation were explored. When treated with kaempferol, the tyrosinase activity and melanin content of PIG1 cells increased, the mRNA and protein expressions of TYR, TRP1, TRP2 and MITF increased, and the phosphorylation level of ERK1\/2 increased. Upon the stimulation of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, kaempferol reduced the production of ROS, decreased apoptosis and increased the protein expression of HO\u20101 in PIG1 cells. In addition, kaempferol inhibited oxidative stress\u2010induced melanin reduction and promoted melanin synthesis in PIG1 cells and protected against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced oxidative stress damage.<\/jats:p>","DOI":"10.1111\/jcmm.17711","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T06:35:22Z","timestamp":1678948522000},"page":"982-990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Kaempferol promotes melanogenesis and reduces oxidative stress in <scp>PIG1<\/scp> normal human skin melanocytes"],"prefix":"10.1111","volume":"27","author":[{"given":"Yihui","family":"Xie","sequence":"first","affiliation":[{"name":"Department of Dermatology, The Affiliated Hospital of Jiaxing University The First Hospital of Jiaxing  Jiaxing 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