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Although the tumorigenesis of FISSs is still uncertain, there is a consensus that FISS is associated with chronic inflammation caused by irritation of injection\u2010related trauma and foreign chemical substances. Chronic inflammation can provide a proper microenvironment for tumour development, which has been known as one of the risk factors of tumorigenesis in many tumours. To investigate the tumorigenesis of FISS and screen for its potential therapeutic targets, cyclooxygenase\u20102 (COX\u20102), an inflammation\u2010enhancing enzyme, was selected as a target for this study. In vitro experiments using FISS\u2010 and normal tissue\u2010derived primary cells and robenacoxib, a highly selective COX\u20102 inhibitor, were performed. The results demonstrated that expression of COX\u20102 could be detected in formalin\u2010fixed and paraffin\u2010embedded FISS tissues and FISS\u2010derived primary cells. Cell viability, migration and colony formation of FISS\u2010derived primary cells were inhibited, and cell apoptosis was enhanced by robenacoxib in a dose\u2010dependent manner. However, susceptibility to robenacoxib varied in different lines of FISS primary cells and was not completely correlated with COX\u20102 expression. Our results suggest that COX\u20102 inhibitors could be potential adjuvant therapeutics against FISSs.<\/jats:p>","DOI":"10.1111\/jcmm.17717","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T09:29:28Z","timestamp":1687166968000},"page":"2183-2193","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of selective cyclooxygenase\u20102 inhibitor robenacoxib on primary cells derived from feline injection\u2010site sarcoma"],"prefix":"10.1111","volume":"27","author":[{"given":"Chen\u2010Hui","family":"Lu","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Graduate Institute of Molecular and Comparative Pathobiology National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010Han","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University Taipei 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