{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T14:16:17Z","timestamp":1784297777734,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","award":["NTU111L893401"],"award-info":[{"award-number":["NTU111L893401"]}],"id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["NTUH\u2010110L\u20101006"],"award-info":[{"award-number":["NTUH\u2010110L\u20101006"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our group have demonstrated that splenic B cells contributed to the CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup> naive T cells conversion into CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>Foxp3<jats:sup>\u2212<\/jats:sup> regulatory T cells without adding appended cytokines, named Treg\u2010of\u2010B cells which were potent suppressors of adaptive immunity. We like to investigate whether Treg\u2010of\u2010B cells could promote alternatively activated macrophage (M2 macrophages) polarization and alleviate inflammatory disease, psoriasis. In this study, we co\u2010cultured the bone marrow\u2010derived macrophages (BMDMs) with Treg\u2010of\u2010B cells under LPS\/IFN\u2010\u03b3 stimulation and analyzed the M2\u2010associated gene and protein using qPCR, western blotting, and immunofluorescence staining. We also examined the therapeutic effect of Treg\u2010of\u2010B cell\u2010induced M2 macrophage for skin inflammation using imiquimod (IMQ)\u2010induced psoriatic mouse model. Our results showed that BMDMs co\u2010cultured with Treg\u2010of\u2010B cells upregulated typical M2\u2010associated molecules, including Arg\u20101, IL\u201010, Pdcd1lg2, MGL\u20101, IL\u20104, YM1\/2 and CD206. In an inflammatory environment, TNF\u2010\u03b1 and IL\u20106 production by macrophages co\u2010cultured with Treg\u2010of\u2010B cells was decreased significantly. The molecular mechanism revealed that Treg\u2010of\u2010B cells promoted M2 macrophage polarization via STAT6 activation in a cell contact\u2010dependent manner. Moreover, the treatment with Treg\u2010of\u2010B cell\u2010induced M2 macrophages attenuated the clinical manifestations of psoriasis, such as scaling, erythema and thickening in the IMQ\u2010induced psoriatic mouse model. T cell activation in draining lymph nodes was decreased in the Treg\u2010of\u2010B cell\u2010induced M2 macrophage group after IMQ application. In conclusion, our findings suggested that Foxp3<jats:sup>\u2212<\/jats:sup> Treg\u2010of\u2010B cells could induce alternatively activated M2 macrophages through STAT6 activation, providing a cell\u2010based therapeutic strategy for psoriasis.<\/jats:p>","DOI":"10.1111\/jcmm.17748","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T08:41:53Z","timestamp":1681893713000},"page":"1477-1492","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["<scp>M2<\/scp>\u2010like macrophages polarized by Foxp3<sup>\u2212<\/sup><scp>Treg\u2010of\u2010B<\/scp> cells ameliorate <scp>imiquimod\u2010induced<\/scp> psoriasis"],"prefix":"10.1111","volume":"27","author":[{"given":"Jing\u2010Hui","family":"Huang","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Li","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Research National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Chieh","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6705-0286","authenticated-orcid":false,"given":"Bor\u2010Luen","family":"Chiang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Department of Medical Research National Taiwan University Hospital  Taipei Taiwan"},{"name":"Department of Pediatrics National Taiwan University Hospital  Taipei Taiwan"},{"name":"Graduate Institute of Immunology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/816460"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00792"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0145342","article-title":"Novel markers to delineate murine M1 and M2 macrophages","volume":"10","author":"Olszewski MA","year":"2015","journal-title":"Plos One"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01625"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0120-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.05.009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2343"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1403225"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.6.2271"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.4.2046"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838201"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2015.05.008"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-053793"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2011-0246OC"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2014.56"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.04.018"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2016.02.002"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32415"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102103"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.20"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.01.006"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2018.05.009"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1994.tb13104.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27180"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22891"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800013"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706832104"},{"key":"e_1_2_9_29_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175336","article-title":"Impact of human monocyte and macrophage polarization on NLR expression and NLRP3 inflammasome activation","volume":"12","author":"Lai HC","year":"2017","journal-title":"Plos One"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.163"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802999"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20161452"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00614"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-016-1037-7"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901081"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-017-0391-3"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.130"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.4.1006"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.5.2253"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.701"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI59643"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.11.009"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-05-285353"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45444"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI29441"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.43"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2007.08.012"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2012.15"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.17748","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T14:59:30Z","timestamp":1692457170000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.17748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,19]]},"references-count":47,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/jcmm.17748"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.17748","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,19]]},"assertion":[{"value":"2022-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}